Rabbit anti-Human, Mouse EDG1 Polyclonal Antibody | anti-EDG1 antibody

EDG1, NT (Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1, Isoform CRA_a, hCG_1640193) (Biotin)

Cat. Num. AAA6473510

• Gene Names: S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EDG1; Polyclonal Antibody; NT (Endothelial Differentiation; Sphingolipid G-protein-coupled Receptor; 1; Isoform CRA_a; hCG_1640193) (Biotin); anti-EDG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EDG1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EDG1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa17-47 of the N-terminal region of human EDG1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hEDG1-I25 in mouse lung tissue lysates (35ug/lane). EDG1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-EDG1 antibody

Antibodies; G Protein Receptors

References

1. Dorsam, G., et al., J. Immunol. 171(7):3500-3507 (2003). 2. zu Heringdorf, D.M., et al., Cell. Signal. 15(7):677-687 (2003). 3. Watterson, K.R., et al., J. Biol. Chem. 277(8):5767-5777 (2002). 4. Liu, Y., et al., J. Clin. Invest. 106(8):951-961 (2000). 5. Lee, M.J., et al., Cell 99(3):301-312 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 13027636
• NCBI GeneID: 1901
• NCBI Accession #: NP_001391
• NCBI GenBank Nucleotide #: NM_001400.4
• UniProt Accession #: P21453; Q9BYY4; Q9NYN8; D3DT66
• Molecular Weight: 42,811 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 1
• NCBI Official Symbol: S1PR1
• NCBI Official Synonym Symbols: EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
• NCBI Protein Information: sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-pr
• UniProt Protein Name: Sphingosine 1-phosphate receptor 1
• UniProt Gene Name: S1PR1
• UniProt Synonym Gene Names: CHEDG1; EDG1; S1P receptor 1; S1P1
• UniProt Entry Name: S1PR1_HUMAN

NCBI Description

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: plasma membrane; integral to membrane; intrinsic to plasma membrane; endosome; external side of plasma membrane; lipid raft

Molecular Function: G-protein coupled receptor activity; sphingolipid binding; G-protein-coupled receptor binding

Biological Process: lamellipodium biogenesis; regulation of cell adhesion; endothelial cell differentiation; regulation of bone resorption; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); cell migration; negative regulation of stress fiber formation; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; transmission of nerve impulse; chemotaxis; blood vessel maturation; neuron differentiation; G-protein coupled receptor protein signaling pathway; actin cytoskeleton reorganization; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, adenylate cyclase inhibiting pathway; brain development; angiogenesis; cell adhesion; regulation of bone mineralization; positive regulation of cell migration