Western Blot (WB)
(All lanes: NIH/3T3 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
NIH/3T3 cell lysate
NIH/3T3
Applications
Western Blot
Synonyms
NIH/3T3; NIH/3T3 cell lysate
Host
Mouse
Form/Format
Liquid; In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue).
Concentration
2mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for NIH/3T3 cell lysate
Western Blot (WB)
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody .
Preparation Before Usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Preparation and Storage
Aliquot the lysate into single tube and then storing them -80 degree C or below. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(All lanes: NIH/3T3 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
Western Blot (WB)
(All lanes: NIH/3T3 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
Related Product Information for NIH/3T3 cell lysate
NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.
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Product Notes
The NIH/3T3 (Catalog #AAA201032) is a Cell Lysate produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NIH/3T3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NIH/3T3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIH/3T3, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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