Human Ubiquitin ELISA Kit | UBB elisa kit

Human Ubiquitin ELISA Kit

Cat. Num. AAA8291390

• Gene Names: UBB; HEL-S-50
• Reactivity: Human
• Synonyms: Ubiquitin; Human Ubiquitin ELISA Kit; Polyubiquitin-B; UBB elisa kit

• Reactivity: Human
• Sequence Length: 229

• Assay Type: Sandwich ELISA
• Samples: Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates
• Detection Range: 125 pg/ml - 8000 pg/ml
• Sensitivity: 60 pg/ml
• Detection Method: Colorimetric
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Representative Standard Curve)

Related Product Information for UBB elisa kit

Sandwich ELISA for quantitative detection of Human Ubiquitin concentrations.

NCBI and Uniprot Product Information

• NCBI GI #: 528524471
• NCBI GeneID: 7314
• NCBI Accession #: NP_001268645.1
• NCBI GenBank Nucleotide #: NM_001281716.1
• UniProt Accession #: P0CG47; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• Molecular Weight: 25,762 Da
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: UBB
• NCBI Official Synonym Symbols: HEL-S-50
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• Protein Family: Ubiquitin
• UniProt Gene Name: UBB

NCBI Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.