Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit | DCLK1 elisa kit

Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit

Cat. Num. AAA2023831

• Gene Names: DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• Reactivity: Human
• Synonyms: Doublecortin Like Kinase 1 (DCLK1); Human Doublecortin Like Kinase 1 (DCLK1) ELISA Kit; DCDC3A; DCLK; DCAMKL1; Doublecortin And CaM Kinase-Like 1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; DCLK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Doublecortin Like Kinase 1 (DCLK1).; No significant cross-reactivity or interference between Doublecortin Like Kinase 1 (DCLK1) and analogues was observed.
• Sequence Length: 433

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Doublecortin Like Kinase 1 (DCLK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DCLK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Doublecortin Like Kinase 1 (DCLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Doublecortin Like Kinase 1 (DCLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Doublecortin Like Kinase 1 (DCLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Doublecortin Like Kinase 1 (DCLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DCLK1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 156230969
• NCBI GeneID: 9201
• UniProt Accession #: O15075; Q5VZY8; Q5VZZ0; Q5VZZ1; B7Z3E9
• Molecular Weight: 47,681 Da
• NCBI Official Full Name: Doublecortin-like kinase 1
• NCBI Official Synonym Full Names: doublecortin-like kinase 1
• NCBI Official Symbol: DCLK1
• NCBI Official Synonym Symbols: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• NCBI Protein Information: serine/threonine-protein kinase DCLK1
• UniProt Protein Name: Serine/threonine-protein kinase DCLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: DCLK1
• UniProt Synonym Gene Names: DCAMKL1; DCDC3A; KIAA0369
• UniProt Entry Name: DCLK1_HUMAN

NCBI Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]

Uniprot Description

DCAMKL1: a serine-threonine kinase of the CAMK family. May be involved in a calcium-signaling pathway controling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. Genetic and physical interactions with Doublecortin, which causes lissencephaly. A putative marker of quiescent stem cells. Four alternatively spliced isoforms have been described.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Nuclear receptor co-regulator; CAMK group; DCAMKL family

Chromosomal Location of Human Ortholog: 13q13

Cellular Component: integral to plasma membrane

Molecular Function: protein serine/threonine kinase activity; receptor signaling protein activity; ATP binding; protein kinase activity

Biological Process: nervous system development; central nervous system development; axon extension; dendrite morphogenesis; response to virus; forebrain development; neuron migration; central nervous system projection neuron axonogenesis; endosome transport; protein amino acid phosphorylation