Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.)
Goat EDD1/HYD Polyclonal Antibody | anti-UBR5 antibody
Goat anti-EDD1/HYD Antibody
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in all germ cells. Recommended concentration, 1-2ug/ml.
Western Blot: An anonymous customer found positive results in WB on Human breast cancer cells (MDA-MB-468). An anonymous customer found positive results in WB on Human HeLa cells. An anonymous customer found positive results in WB on Human glioblastoma (T98G cells). An anonymous customer found positive results in WB on Rat glioma (C6 cells). An anonymous customer found positive results in WB on Mouse fibroblasts (NIH 3T3). An anonymous customer found positive results in WB on Rabbit heart tissue.
Immunoprecipitation: An anonymous customer found positive results in IP on Human breast cancer cells. IP; An anonymous customer found positive results in IP on Mouse lung tissue. IP; An anonymous customer found positive results in IP on Rat lung tissue. IP; An anonymous customer found positive results in IP on Rabbit heart tissue.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Nuclear receptor co-regulator; Ligase
Chromosomal Location of Human Ortholog: 8q22
Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus
Molecular Function: ligase activity; protein binding; RNA binding; ubiquitin binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: cell proliferation; DNA repair; positive regulation of protein import into nucleus, translocation; progesterone receptor signaling pathway; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to DNA damage stimulus; ubiquitin-dependent protein catabolic process
Research Articles on UBR5
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Product Notes
The UBR5 ubr5 (Catalog #AAA420631) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EDD1/HYD Antibody reacts with Tested: Human, Rat, Mouse, Rabbit; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's EDD1/HYD can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in all germ cells. Recommended concentration, 1-2ug/ml. Western Blot: An anonymous customer found positive results in WB on Human breast cancer cells (MDA-MB-468). An anonymous customer found positive results in WB on Human HeLa cells. An anonymous customer found positive results in WB on Human glioblastoma (T98G cells). An anonymous customer found positive results in WB on Rat glioma (C6 cells). An anonymous customer found positive results in WB on Mouse fibroblasts (NIH 3T3). An anonymous customer found positive results in WB on Rabbit heart tissue. Immunoprecipitation: An anonymous customer found positive results in IP on Human breast cancer cells. IP; An anonymous customer found positive results in IP on Mouse lung tissue. IP; An anonymous customer found positive results in IP on Rat lung tissue. IP; An anonymous customer found positive results in IP on Rabbit heart tissue. Researchers should empirically determine the suitability of the UBR5 ubr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LAIKTKNFGF V. It is sometimes possible for the material contained within the vial of "EDD1/HYD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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