Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Rabbit anti-Human ECT2 Polyclonal Antibody | anti-Ect2 antibody
ECT2, ID (ECT2, Protein ECT2, Epithelial cell-transforming sequence 2 oncogene)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Immunohistochemistry (IHC)
(ECT2 Antibody (Center) (AP14755c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ECT2 Antibody (Center) (AP14755c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. Ref.8 Ref.9
Subunit structure: Homodimer. Homooligomer. Found in the centralspindlin complex. Interacts (Thr-359 phosphorylated form) with PARD6A; the interaction is observed in cancer cells. Interacts (Thr-359 phosphorylated form) with PRKCI; the interaction is observed in cancer cells. Interacts with PKP4; the interaction is observed at the midbody. Interacts with RACGAP1; the interaction is direct, occurs in a microtubule-dependent manner, is inhibited in metaphase by phosphorylation of ECT2 on Thr-373 and is stimulated in early anaphase by dephosphorylation of ECT2 probably on Thr-373 through CDK1 activity. Interacts with PLK1; the interaction is stimulated upon its phosphorylation on Thr-444. Associates with RACGAP1 at anaphase and during cytokinesis. Interacts with KIF23, PARD3, PARD6A, PARD6B and PRKCQ
By similarity. Interacts with NR1I3. Ref.8
Subcellular location: Nucleus. Cytoplasm. Cytoplasm › cytoskeleton › spindle
By similarity. Cleavage furrow
By similarity. Midbody
By similarity. Cell junction
By similarity. Cell junction › tight junction
By similarity. Note: Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells
By similarity. Ref.8
Tissue specificity: Highest expression in testis. Also detectable in brain, kidney, liver and spleen. Ref.9
Developmental stage: Expressed in the embryo at 16 dpc. Ref.9
Induction: Up-regulated by phenobarbital in the nucleus and cytoplasm of the liver. Ref.8
Domain: The BRCT domain 1 and 2 are required for the intramolecular interaction, but not for the intermolecular oligomerization. The BRCT domains negatively inhibit its GEF activity in interphase cells. The same BRCT domains may act as a positive regulatory motif for the completion of cytokinesis after the breakdown of nuclear membrane during mitosis
By similarity.
Post-translational modification: Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells
By similarity.
Sequence similarities: Contains 2 BRCT domains.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.
Sequence caution: The sequence AAA37536.1 differs from that shown. Reason: Erroneous CDS prediction.
Research Articles on Ect2
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Product Notes
The Ect2 ect2 (Catalog #AAA646444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECT2, ID (ECT2, Protein ECT2, Epithelial cell-transforming sequence 2 oncogene) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the Ect2 ect2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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