Typical Testing Data/Standard Curve (for reference only)
Mouse Sulfiredoxin-1 ELISA Kit | Srxn1 elisa kit
Mouse Sulfiredoxin-1 ELISA Kit
Gene Names
Srxn1; Srx; Npn3; Srx1; TX01; AI854065; AW488194; 1700127B04Rik
Reactivity
Mouse
Synonyms
Sulfiredoxin-1; Mouse Sulfiredoxin-1 ELISA Kit; Neoplastic progression protein 3; Srxn1; Npn3; Srx; 1.8.98.2; Srxn1 elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse Sulfiredoxin-1. No significant cross-reactivity or interference was observed.
Sequence Length
155
Assay Type
Sandwich
Detection Range
0.156-10 ng/mL
Sensitivity
0.078 ng/mL
Intra-Assay CV
<=5.2%
Inter-Assay CV
<=10.2%
Recovery
86%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Srxn1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Sulfiredoxin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfiredoxin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Sulfiredoxin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sulfiredoxin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sulfiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfiredoxin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Sulfiredoxin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sulfiredoxin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sulfiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,149 Da
NCBI Official Full Name
sulfiredoxin-1
NCBI Official Synonym Full Names
sulfiredoxin 1 homolog (S. cerevisiae)
NCBI Official Symbol
Srxn1
NCBI Official Synonym Symbols
Srx; Npn3; Srx1; TX01; AI854065; AW488194; 1700127B04Rik
NCBI Protein Information
sulfiredoxin-1
UniProt Protein Name
Sulfiredoxin-1
Protein Family
UniProt Gene Name
Srxn1
UniProt Synonym Gene Names
Npn3; Srx
UniProt Entry Name
SRXN1_MOUSE
Uniprot Description
SRXN1: Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase. Belongs to the sulfiredoxin family.
Protein type: Oxidoreductase; EC 1.8.98.2
Cellular Component: cytoplasm; cytosol
Molecular Function: antioxidant activity; ATP binding; nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors; sulfiredoxin activity
Biological Process: response to oxidative stress
Research Articles on Srxn1
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Product Notes
The Mouse Srxn1 srxn1 (Catalog #AAA289502) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289502 ELISA Kit recognizes Mouse Srxn1. It is sometimes possible for the material contained within the vial of "Sulfiredoxin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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