Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Rabbit ECT2 Polyclonal Antibody | anti-ECT2 antibody
ECT2 Antibody (Center)
Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Western Blot (WB)
(ECT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ECT2 antibody detected the ECT2 protein (arrow).)
Immunohistochemistry (IHC)
(ECT2 Antibody (Center) (MBS9206215)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ECT2 Antibody (Center) (MBS9206215)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
that is related to Rho-specific exchange factors and yeast cell
cycle regulators. The expression of this gene is elevated with the
onset of DNA synthesis and remains elevated during G2 and M phases.
In situ hybridization analysis showed that expression is at a high
level in cells undergoing mitosis in regenerating liver. Thus, this
protein is expressed in a cell cycle-dependent manner during liver
regeneration, and is thought to have an important role in the
regulation of cytokinesis.
Burkard, M.E., et al. PLoS Biol. 7 (5), E1000111 (2009) :
Wolfe, B.A., et al. PLoS Biol. 7 (5), E1000110 (2009) :
Hirata, D., et al. Clin. Cancer Res. 15(1):256-266(2009)
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
ECT2: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 3q26.1-q26.2
Cellular Component: nuclear membrane; tight junction; intracellular membrane-bound organelle; cytoplasm; midbody; intercellular junction; nucleus; cytosol; cleavage furrow
Molecular Function: signal transducer activity; protein binding; Rho guanyl-nucleotide exchange factor activity; Rho GTPase binding; protein homodimerization activity; GTPase activator activity
Biological Process: positive regulation of cytokinesis; regulation of attachment of spindle microtubules to kinetochore; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; cell morphogenesis; cytokinesis; protein transport; regulation of small GTPase mediated signal transduction; positive regulation of protein import into nucleus; small GTPase mediated signal transduction; activation of protein kinase activity; regulation of protein kinase activity; positive regulation of neuron differentiation; protein homooligomerization
Research Articles on ECT2
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Product Notes
The ECT2 ect2 (Catalog #AAA9206215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 597-625. The ECT2 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ECT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the ECT2 ect2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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