Rabbit anti-Human ECHS1 Polyclonal Antibody | anti-ECHS1 antibody

Anti-ECHS1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107485

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ECHS1; Polyclonal Antibody; Anti-ECHS1 Antibody; Rabbit Polyclonal; ECHS1 Antibody; Rabbit PAb; Antigen Affinity Purified; enoyl CoA hydratase; short chain; 1; mitochondrial; Anti-ECHS1D Antibody; Anti-SCEH Antibody; anti-ECHS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ECHS1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ECHS1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 4-8uL/mg of lysate
• Immunogen: Recombinant Human ECHS1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ECHS1 (rh ECHS1; AAH08906.1; 28A-290Q). ECHS1 specific IgG was purified by Human ECHS1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(ECHS1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ECHS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-ECHS1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)

Western Blot (WB)

(Anti-ECHS1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: 293T Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-ECHS1 antibody

Overexpression of ECHS1 occurs in different cancers, including hepatocellular carcinoma (HCC). The coexistence of HBs and ECHS1 enhances HepG2 cell apoptosis, affects ECHS1 localization in the mitochondria and induces apoptosis by decreasing the mitochondrial membrane potential (MMP). These findings suggest that ECHS1 may be applied as a potential therapeutic target during the treatment of HBV-related hepatitis or HCC. The human ECHS1 gene encodes the short-chain enoyl coenzyme A hydratase, the enzyme that catalyzes the second step of beta-oxidation of fatty acids in the mitochondrial matrix. ECHS1 protein expression was severely depleted in the patient's skeletal muscle and patient-derived myoblasts. Short-chain enoyl-CoA hydratase (ECHS1) is a multifunctional mitochondrial matrix enzyme that is involved in the oxidation of fatty acids and essential amino acids such as valine. Silencing ECHS1 to attenuate proliferation and induce autophagy may make it a novel cancer therapy target.