SDS-Page
ECHS1 active protein
ECHS1, human recombinant
Gene Names
ECHS1; SCEH
Purity
>95% by SDS-PAGE
Synonyms
ECHS1; human recombinant; Enoyl Coenzyme A hydratase; short chain 1 mitochondrial; SCEH; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; ECHS1 active protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl
Sequence
MGSSHHHHHHSSGLVPRGSHMASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFaaGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
Gene Source
Human
Biological Activity
Specific activity > 150 units/mg
Measured by its ability to hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25 degree C.
Measured by its ability to hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25 degree C.
Preparation and Storage
Store at -20 degree C
Centrifuge the vial prior to opening.
Shelf life: ~12 months
Centrifuge the vial prior to opening.
Shelf life: ~12 months
SDS-Page
SDS-Page
Related Product Information for ECHS1 active protein
Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ECHS1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
290
NCBI Official Full Name
enoyl-CoA hydratase, mitochondrial
NCBI Official Synonym Full Names
enoyl CoA hydratase, short chain, 1, mitochondrial
NCBI Official Symbol
ECHS1
NCBI Official Synonym Symbols
SCEH
NCBI Protein Information
enoyl-CoA hydratase, mitochondrial; enoyl-CoA hydratase 1; short-chain enoyl-CoA hydratase; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
UniProt Protein Name
Enoyl-CoA hydratase, mitochondrial
Protein Family
UniProt Gene Name
ECHS1
UniProt Synonym Gene Names
SCEH
UniProt Entry Name
ECHM_HUMAN
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Product Notes
The ECHS1 echs1 (Catalog #AAA849818) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFaa GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ. It is sometimes possible for the material contained within the vial of "ECHS1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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