Rabbit anti-Human ECAT1 Polyclonal Antibody | anti-ECAT1 antibody

ECAT1, NT (ECAT1, C6orf221, ES cell-associated transcript 1 protein) (FITC)

Cat. Num. AAA6294217

• Gene Names: KHDC3L; ECAT1; HYDM2; C6orf221
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ECAT1; Polyclonal Antibody; NT (ECAT1; C6orf221; ES cell-associated transcript 1 protein) (FITC); ES cell-associated transcript 1 protein; anti-ECAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ECAT1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human ECAT1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ECAT1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).)

Immunohistochemistry (IHC)

(ECAT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECAT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ECAT1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ECAT1 antibody

Antibodies; KIAA; orf

References

Pierre, A., et al. Genomics 90(5):583-594(2007). Mitsui, K., et al. Cell 113(5):631-642(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 62821796
• NCBI GeneID: 154288
• NCBI Accession #: NP_001017361
• NCBI GenBank Nucleotide #: NM_001017361.2
• UniProt Accession #: Q587J8; B2RNW7
• Molecular Weight: 24,306 Da
• NCBI Official Full Name: KHDC3-like protein
• NCBI Official Synonym Full Names: KH domain containing 3 like, subcortical maternal complex member
• NCBI Official Symbol: KHDC3L
• NCBI Official Synonym Symbols: ECAT1; HYDM2; C6orf221
• NCBI Protein Information: KHDC3-like protein
• UniProt Protein Name: KHDC3-like protein
• UniProt Gene Name: KHDC3L
• UniProt Synonym Gene Names: C6orf221; ECAT1
• UniProt Entry Name: KHD3L_HUMAN

NCBI Description

The protein encoded by this gene belongs to the KHDC1 family, members of which contain an atypical KH domain that may not bind RNA like canonical KH domains. This gene is specifically expressed in the oocytes, and recent studies suggest that it may function as a regulator of genomic imprinting in the oocyte. Mutations in this gene are associated with recurrent biparental complete hydatidiform mole. [provided by RefSeq, Dec 2011]

Uniprot Description

KHDC3L: Defects in KHDC3L are the cause of hydatidiform mole recurrent type 2 (HYDM2). A disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the KHDC1 family.

Chromosomal Location of Human Ortholog: 6q13

Molecular Function: RNA binding

Disease: Hydatidiform Mole, Recurrent, 2