Rabbit anti-Human ECAT1 Polyclonal Antibody | anti-KHDC3L antibody

ECAT1, NT (ECAT1, C6orf221, ES cell-associated transcript 1 protein)

Cat. Num. AAA642518

• Gene Names: KHDC3L; ECAT1; HYDM2; C6orf221
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ECAT1; Polyclonal Antibody; NT (ECAT1; C6orf221; ES cell-associated transcript 1 protein); Anti -ECAT1; anti-KHDC3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-KHDC3L antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human ECAT1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ECAT1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).)

Immunohistochemistry (IHC)

(ECAT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECAT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ECAT1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KHDC3L antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 119569158
• NCBI GeneID: 154288
• UniProt Accession #: Q587J8; B2RNW7
• Molecular Weight: 24,306 Da
• NCBI Official Full Name: ES cell associated trasnsript 1
• NCBI Official Synonym Full Names: KH domain containing 3-like, subcortical maternal complex member
• NCBI Official Symbol: KHDC3L
• NCBI Official Synonym Symbols: ECAT1; HYDM2; C6orf221
• NCBI Protein Information: KHDC3-like protein; ES cell-associated transcript 1 protein
• UniProt Protein Name: KHDC3-like protein
• Protein Family: KHDC3-like protein
• UniProt Gene Name: KHDC3L
• UniProt Synonym Gene Names: C6orf221; ECAT1
• UniProt Entry Name: KHD3L_HUMAN

NCBI Description

The protein encoded by this gene belongs to the KHDC1 family, members of which contain an atypical KH domain that may not bind RNA like canonical KH domains. This gene is specifically expressed in the oocytes, and recent studies suggest that it may function as a regulator of genomic imprinting in the oocyte. Mutations in this gene are associated with recurrent biparental complete hydatidiform mole. [provided by RefSeq, Dec 2011]

Uniprot Description

KHDC3L: Defects in KHDC3L are the cause of hydatidiform mole recurrent type 2 (HYDM2). A disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the KHDC1 family.

Chromosomal Location of Human Ortholog: 6q13

Molecular Function: RNA binding

Disease: Hydatidiform Mole, Recurrent, 2