Human Phosphoserine Phosphatase (PSPH) CLIA Kit | PSPH clia kit

Human Phosphoserine Phosphatase (PSPH) CLIA Kit

Cat. Num. AAA2001078

• Gene Names: PSPH; PSP; PSPHD
• Reactivity: Human
• Synonyms: Phosphoserine Phosphatase (PSPH); Human Phosphoserine Phosphatase (PSPH) CLIA Kit; PSP; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; PSPH clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoserine Phosphatase (PSPH).; No significant cross-reactivity or interference between Phosphoserine Phosphatase (PSPH) and analogues was observed.
• Sequence Length: 225

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: < 5.7pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSPH clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Phosphoserine Phosphatase (PSPH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Phosphoserine Phosphatase (PSPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phosphoserine Phosphatase (PSPH) level in the sample or standard.;

Product Categories/Family for PSPH clia kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 46249388
• NCBI GeneID: 5723
• NCBI Accession #: NP_004568.2
• NCBI GenBank Nucleotide #: NM_004577.3
• UniProt Accession #: P78330; Q7Z3S5; B2RCR5
• Molecular Weight: 25,008 Da
• NCBI Official Full Name: phosphoserine phosphatase
• NCBI Official Synonym Full Names: phosphoserine phosphatase
• NCBI Official Symbol: PSPH
• NCBI Official Synonym Symbols: PSP; PSPHD
• NCBI Protein Information: phosphoserine phosphatase
• UniProt Protein Name: Phosphoserine phosphatase
• UniProt Gene Name: PSPH
• UniProt Synonym Gene Names: PSP; PSPase
• UniProt Entry Name: SERB_HUMAN

NCBI Description

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSPH: Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. Defects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome. Belongs to the SerB family.

Protein type: Motility/polarity/chemotaxis; Hydrolase; Amino Acid Metabolism - glycine, serine and threonine; EC 3.1.3.3

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: neuron projection; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; phosphoserine phosphatase activity; magnesium ion binding; calcium ion binding

Biological Process: response to nutrient levels; L-serine biosynthetic process; dephosphorylation; response to mechanical stimulus; L-serine metabolic process; response to testosterone stimulus; amino acid biosynthetic process

Disease: Phosphoserine Phosphatase Deficiency