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Western Blot (WB) (ECAT1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).)

Rabbit anti-Human ECAT1 Polyclonal Antibody | anti-KHDC3L antibody

ECAT1 Antibody (N-term)

Gene Names
KHDC3L; ECAT1; HYDM2; C6orf221
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ECAT1; Polyclonal Antibody; ECAT1 Antibody (N-term); KHDC3-like protein; ES cell-associated transcript 1 protein; KHDC3L; C6orf221; anti-KHDC3L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human ECAT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.39 (varies by lot)
Sequence Positions
20-48
Sequence Length
217
Applicable Applications for anti-KHDC3L antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ECAT1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).)

Western Blot (WB) (ECAT1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ECAT1 antibody detected the ECAT1 protein (arrow).)

Immunohistochemistry (IHC)

(ECAT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECAT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ECAT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ECAT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ECAT1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ECAT1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-KHDC3L antibody
References
Pierre, A., et al. Genomics 90(5):583-594(2007) Mitsui, K., et al. Cell 113(5):631-642(2003)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24306
NCBI Official Full Name
KHDC3-like protein
NCBI Official Synonym Full Names
KH domain containing 3-like, subcortical maternal complex member
NCBI Official Symbol
KHDC3L
NCBI Official Synonym Symbols
ECAT1; HYDM2; C6orf221
NCBI Protein Information
KHDC3-like protein
UniProt Protein Name
KHDC3-like protein
Protein Family
UniProt Gene Name
KHDC3L
UniProt Synonym Gene Names
C6orf221; ECAT1
UniProt Entry Name
KHD3L_HUMAN

NCBI Description

The protein encoded by this gene belongs to the KHDC1 family, members of which contain an atypical KH domain that may not bind RNA like canonical KH domains. This gene is specifically expressed in the oocytes, and recent studies suggest that it may function as a regulator of genomic imprinting in the oocyte. Mutations in this gene are associated with recurrent biparental complete hydatidiform mole. [provided by RefSeq, Dec 2011]

Uniprot Description

KHDC3L: Defects in KHDC3L are the cause of hydatidiform mole recurrent type 2 (HYDM2). A disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the KHDC1 family.

Chromosomal Location of Human Ortholog: 6q13

Molecular Function: RNA binding

Disease: Hydatidiform Mole, Recurrent, 2

Research Articles on KHDC3L

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Product Notes

The KHDC3L khdc3l (Catalog #AAA9214659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-48. The ECAT1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECAT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the KHDC3L khdc3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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