Typical Testing Data/Standard Curve (for reference only)
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Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1 ELISA Kit | PLCB1 elisa kit
Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PLCB1 antibody. PLCB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLCB1 Antibody is added and binds to PLCB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLCB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLCB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PLCB1: an enzyme that catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Binds to calmodulin. Uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variant isoforms have been described.
Protein type: Phospholipase; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 20p12
Cellular Component: nuclear membrane; nuclear chromatin; cytoplasm; nuclear speck; cytosol; nucleus
Molecular Function: calmodulin binding; signal transducer activity; phosphatidylinositol-4,5-bisphosphate binding; enzyme binding; protein homodimerization activity; calcium ion binding; phosphoinositide phospholipase C activity; GTPase activator activity; lamin binding
Biological Process: fat cell differentiation; positive regulation of developmental growth; inositol phosphate metabolic process; acetylcholine receptor signaling, muscarinic pathway; glutamate signaling pathway; metabolic process; positive regulation of interleukin-12 production; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; signal transduction; positive regulation of myoblast differentiation; memory; synaptic transmission; positive regulation of embryonic development; insulin-like growth factor receptor signaling pathway; cerebral cortex development; phosphatidylinositol metabolic process; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of G-protein coupled receptor protein signaling pathway; lipid catabolic process; positive regulation of GTPase activity
Disease: Epileptic Encephalopathy, Early Infantile, 12
Research Articles on PLCB1
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Product Notes
The Human PLCB1 plcb1 (Catalog #AAA1605818) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1605818 ELISA Kit recognizes Human PLCB1. It is sometimes possible for the material contained within the vial of "1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-1, PLCB1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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