Western Blot (WB)
(Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: K562)
Rabbit EB3 Monoclonal Antibody | anti-EB3 antibody
EB3 Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: K562)
Western Blot (WB)
(Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue Lane 2: K562)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq, Jul 2008]
Uniprot Description
EB3: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. Belongs to the MAPRE family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Microtubule-binding
Chromosomal Location of Human Ortholog: 2p23.3-p23.1
Cellular Component: microtubule cytoskeleton; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; midbody
Molecular Function: protein binding; microtubule binding
Biological Process: mitosis; positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of transcription, DNA-dependent
Research Articles on EB3
Similar Products
Product Notes
The EB3 mapre3 (Catalog #AAA9436523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EB3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EB3 mapre3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EB3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
