Rabbit anti-Mouse E-Cadherin Polyclonal Antibody | anti-CDH1 antibody

Anti-E-Cadherin Antibody, Rabbit Polyclonal

Cat. Num. AAA8111899

• Gene Names: Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: E-Cadherin; Polyclonal Antibody; Anti-E-Cadherin Antibody; Rabbit Polyclonal; E-Cadherin/CDH1/E-cad/CD324 Antibody; Rabbit PAb; Antigen Affinity Purified; cadherin 1; type 1; E-cadherin (epithelial); Anti-AA960649 Antibody; Anti-ARC-1 Antibody; Anti-E-cad Antibody; Anti-Ecad Antibody; Anti-L-CAM Antibody; Anti-Um Antibody; Anti-UVO Antibody; anti-CDH1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse E-Cadherin/CDH1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDH1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Mouse E-Cadherin/CDH1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin/CDH1 (rM E-Cadherin/CDH1). E-Cadherin/CDH1 specific IgG was purified by Mouse E-Cadherin/CDH1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CDH1 in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CDH1 in mouse liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunoprecipitation (IP)

(Mouse CDH1 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-Mouse CDH1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CDH1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 130 kDaObserved band size: 130 kDa)

Western Blot (WB)

(Anti-CDH1 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: MDA-MB-468 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:97 kDaObserved band size:150 kDa)

Related Product Information for anti-CDH1 antibody

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Product Categories/Family for anti-CDH1 antibody

Hemangioblast Markers Tumor Suppressors; Canonical Wnt Pathway

References

Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.

NCBI and Uniprot Product Information

• NCBI GI #: 115419
• NCBI GeneID: 12550
• UniProt Accession #: P09803; Q61377
• Molecular Weight: 884
• NCBI Official Full Name: Cadherin-1
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: Cdh1
• NCBI Official Synonym Symbols: Um; UVO; Ecad; E-cad; L-CAM; AA960649
• NCBI Protein Information: cadherin-1; ARC-1; E-cadherin; uvomorulin; epithelial cadherin
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: Cdh1
• UniProt Synonym Gene Names: E-cadherin
• UniProt Entry Name: CADH1_MOUSE

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Ubiquitin ligase

Cellular Component: apical junction complex; Golgi apparatus; internal side of plasma membrane; cell surface; focal adhesion; basolateral plasma membrane; lateral loop; integral to membrane; catenin complex; trans-Golgi network; intercellular junction; actin cytoskeleton; cell-cell adherens junction; adherens junction; membrane; apical part of cell; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; nerve terminal; cell junction; endosome; lateral plasma membrane

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; metal ion binding; beta-catenin binding; GTPase activating protein binding; calcium ion binding; ankyrin binding; cell adhesion molecule binding; glycoprotein binding; protein phosphatase binding

Biological Process: in utero embryonic development; positive regulation of transcription, DNA-dependent; regulation of caspase activity; regulation of epithelial cell proliferation; trophectodermal cell differentiation; regulation of water loss via skin; cell-cell adhesion; sensory perception of sound; regulation of protein localization; synaptogenesis; protein metabolic process; calcium-dependent cell-cell adhesion; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; cell adhesion; homophilic cell adhesion; protein homooligomerization; negative regulation of epithelial cell proliferation