Rabbit anti-Human, Mouse Dyrk2 Polyclonal Antibody | anti-DYRK2 antibody

Dyrk2, CT (Dyrk2, Dual specificity tyrosine-phosphorylation-regulated kinase 2)

Cat. Num. AAA649925

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Dyrk2; Polyclonal Antibody; CT (Dyrk2; Dual specificity tyrosine-phosphorylation-regulated kinase 2); Anti -Dyrk2; anti-DYRK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DYRK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Dyrk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-549 amino acids from the C-terminal region of human Mouse Dyrk2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Dyrk2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Dyrk2 antibody detected the Dyrk2 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Dyrk2 Antibody (C-term) (AP14070b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Dyrk2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DYRK2 antibody

Role in the regulation of cellular growth and/or development. Regulates TP53 by phosphorylation on Ser-46 to induce apoptosis in response to DNA damage, functioning downstream of ATM. Inactivates GYS1 by phosphorylation at Ser-641, and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates (By similarity).

Product Categories/Family for anti-DYRK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 153281169
• NCBI GeneID: 8445
• NCBI Accession #: NP_006473.2
• NCBI GenBank Nucleotide #: NM_006482.2
• UniProt Accession #: Q92630; Q9BRB5; B2R9V9
• Molecular Weight: 66,652 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 2 isoform 2
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
• NCBI Official Symbol: DYRK2
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 2
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 2
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK2
• UniProt Entry Name: DYRK2_HUMAN

NCBI Description

DYRK2 belongs to a family of protein kinases whose members are presumed to be involved in cellular growth and/or development. The family is defined by structural similarity of their kinase domains and their capability to autophosphorylate on tyrosine residues. DYRK2 has demonstrated tyrosine autophosphorylation and catalyzed phosphorylation of histones H3 and H2B in vitro. Two isoforms of DYRK2 have been isolated. The predominant isoform, isoform 1, lacks a 5' terminal insert. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Serine/threonine-protein kinase involved in the regulation of the mitotic cell cycle, cell proliferation, apoptosis, organization of the cytoskeleton and neurite outgrowth. Functions in part via its role in ubiquitin-dependent proteasomal protein degradation. Functions downstream of ATM and phosphorylates p53/TP53 at 'Ser-46', and thereby contributes to the induction of apoptosis in response to DNA damage. Phosphorylates NFATC1, and thereby inhibits its accumulation in the nucleus and its transcription factor activity. Phosphorylates EIF2B5 at 'Ser-544', enabling its subsequent phosphorylation and inhibition by GSK3B. Likewise, phosphorylation of NFATC1, CRMP2/DPYSL2 and CRMP4/DPYSL3 promotes their subsequent phosphorylation by GSK3B. May play a general role in the priming of GSK3 substrates. Inactivates GYS1 by phosphorylation at 'Ser-641', and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Mediates EDVP E3 ligase complex formation and is required for the phosphorylation and subsequent degradation of KATNA1. Phosphorylates SIAH2, and thereby increases its ubiquitin ligase activity. Promotes the proteasomal degradation of MYC and JUN, and thereby regulates progress through the mitotic cell cycle and cell proliferation. Promotes proteasomal degradation of GLI2 and GLI3, and thereby plays a role in smoothened and sonic hedgehog signaling. Plays a role in cytoskeleton organization and neurite outgrowth via its phosphorylation of DCX and DPYSL2. Phosphorylates CRMP2/DPYSL2, CRMP4/DPYSL3, DCX, EIF2B5, EIF4EBP1, GLI2, GLI3, GYS1, JUN, MDM2, MYC, NFATC1, p53/TP53, TAU/MAPT and KATNA1. Can phosphorylate histone H1, histone H3 and histone H2B (in vitro). Can phosphorylate CARHSP1 (in vitro). Ref.1 Ref.6 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.19 Ref.22 Ref.23

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium. Ref.1Manganese. Ref.1

Enzyme regulation: Activated by autophosphorylation on the second tyrosine residue in the Tyr-X-Tyr motif in the activation loop. Inhibited by acridine analogs, purvalanol, and barely by harmine. Inhibited by leucettine and leucettine derivatives. Ref.12 Ref.18 Ref.20 Ref.25

Subunit structure: Component of an E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP (EDVP complex). Interacts directly with EDD/UBR5, DDB1 and VPRBP. Interacts with SIAH2 and MDM2. Interacts with MAP3K10 and NFATC1. May also interact with CCNL2. Ref.10 Ref.13 Ref.19 Ref.21

Subcellular location: Cytoplasm. Nucleus. Note: Translocates into the nucleus following DNA damage. Ref.14 Ref.15 Ref.17 Ref.21

Tissue specificity: Testis, after the onset of spermatogenesis. Ref.1

Induction: Accumulates in nucleus upon DNA damage. Induced in both esophageal and lung adenocarcinomas. Ref.7 Ref.12 Ref.18 Ref.20 Ref.21 Ref.25

Post-translational modification: Autophosphorylates cotranslationally on the second tyrosine residue in the Tyr-X-Tyr motif in the activation loop, but once mature, does not have any protein tyrosine kinase activity. Phosphorylated at Thr-106 and Ser-442 by ATM in response to genotoxic stress. Ref.1 Ref.15 Ref.16 Ref.21 Ref.25Under normal conditions, polyubiquitinated in the nucleus by MDM2, leading to its proteasomal degradation. Phosphorylation on Thr-106 and Ser-442 by ATM in response to genotoxic stress disrupts MDM2 binding and prevents MDM2-mediated ubiquitination and subsequent proteasomal degradation. Polyubiquitinated by SIAH2, leading to its proteasomal degradation. Polyubiquitinated by SIAH2 occurs under normal conditions, and is enhanced in response to hypoxia.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence CAA73885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.