Rabbit DUSP16 / MKP7 Polyclonal Antibody | anti-DUSP16 antibody

Rabbit Polyclonal to Human DUSP16 / MKP7

Cat. Num. AAA241683

• Gene Names: DUSP16; MKP7; MKP-7
• Reactivity: Bovine, Chimpanzee, Chicken, Human, Monkey, Mouse, Zebrafish
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: DUSP16 / MKP7; Polyclonal Antibody; Rabbit Polyclonal to Human DUSP16 / MKP7; Anti-DUSP16 / MKP7 Antibody (aa600-650) IHC-plus; DUSP16; MAPK phosphatase-7; MKP7; MAP kinase phosphatase 7; MKP-7; KIAA1700; Human DUSP16; anti-DUSP16 antibody

• Host: Rabbit
• Reactivity: Bovine, Chimpanzee, Chicken, Human, Monkey, Mouse, Zebrafish
• Clonality: Polyclonal
• Specificity: A portion of amino acids 600-650 of human DUSP16
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.2% gelatin, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 665

• Applicable Applications for anti-DUSP16 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human DUSP16.
• Immunogen Type: Synthetic peptide
• Immunogen: DUSP16 / MKP7 antibody was raised against synthetic peptide from human DUSP16.
• Antigen Modification: aa600-650
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-DUSP16 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of human DUSP16? in Jurkat cell lysate using antibody at 2 ug/ml.)

Related Product Information for anti-DUSP16 antibody

MKP7 is a dual specificity MAP kinase phosphatase which has been shown to bind and inactivate kinases p38 (isoforms alpha and beta) and JNK, but not ERK. A mutant form MKP7 has been shown to function as a dominant negative, particularly against the dephosphorylation of JNK in vitro, suggesting that MKP7 works as a JNK-specific phosphatase in vivo. Detailed transcriptional mapping of 12p12 chromosomal region has identified MKP7 as a possible tumor suppressor gene.

Product Categories/Family for anti-DUSP16 antibody

Family: Protein Phosphatase; Subfamily: Dual specificity MKP

NCBI and Uniprot Product Information

• NCBI GI #: 38372911
• NCBI GeneID: 80824
• NCBI Accession #: NP_085143.1
• NCBI GenBank Nucleotide #: NM_030640.2
• UniProt Accession #: Q9BY84; Q547C7; Q96QS2; Q9C0G3
• Molecular Weight: 16,078 Da
• NCBI Official Full Name: dual specificity protein phosphatase 16
• NCBI Official Synonym Full Names: dual specificity phosphatase 16
• NCBI Official Symbol: DUSP16
• NCBI Official Synonym Symbols: MKP7; MKP-7
• NCBI Protein Information: dual specificity protein phosphatase 16; MAPK phosphatase-7; MAP kinase phosphatase 7; mitogen-activated protein kinase phosphatase 7
• UniProt Protein Name: Dual specificity protein phosphatase 16
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP16
• UniProt Synonym Gene Names: KIAA1700; MKP7; MAP kinase phosphatase 7; MKP-7
• UniProt Entry Name: DUS16_HUMAN

NCBI Description

This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]

Uniprot Description

MKP-7: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP16 suppresses MAPK activation in the order of selectivity, JNK >> p38 > ERK, but interacts with ERK as well as JNK and p38. Overexpression of DUSP16 in BCR-ABL-transformed fibroblasts reduces their transforming capacity in vitro and in vivo via down regulation of BCR-ABL-induced JNK activation. DUSP16 might be haploinsufficient for tumor suppression. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleoplasm; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: MAPK export from nucleus; dephosphorylation; MAPK phosphatase export from nucleus, leptomycin B sensitive; protein amino acid dephosphorylation; inactivation of MAPK activity