Rabbit anti-Human DUSP10 Polyclonal Antibody | anti-DUSP10 antibody

DUSP10, ID (DUSP10, MKP5, Dual specificity protein phosphatase 10, Mitogen-activated protein kinase phosphatase 5) (Azide free) (HRP)

Cat. Num. AAA6293767

• Gene Names: DUSP10; MKP5; MKP-5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUSP10; Polyclonal Antibody; ID (DUSP10; MKP5; Dual specificity protein phosphatase 10; Mitogen-activated protein kinase phosphatase 5) (Azide free) (HRP); Mitogen-activated protein kinase phosphatase 5; anti-DUSP10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DUSP10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUSP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the Central region of human DUSP10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUSP10 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the DUSP10 antibody detected the DUSP10 protein (arrow).)

Related Product Information for anti-DUSP10 antibody

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-DUSP10 antibody

Antibodies; Protein Kinases

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Tephly, L.A., et al. Am. J. Respir. Cell Mol. Biol. 39(1):113-123(2008). Teng, C.H., et al. J. Biol. Chem. 282(39):28395-28407(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21536333
• NCBI GeneID: 11221
• NCBI Accession #: NP_653330.1
• NCBI GenBank Nucleotide #: NM_144729.2
• UniProt Accession #: Q9Y6W6
• Molecular Weight: 40.4 kDa (359aa), confirmed by MALDI-TOF
• NCBI Official Synonym Full Names: dual specificity phosphatase 10
• NCBI Official Symbol: DUSP10
• NCBI Official Synonym Symbols: MKP5; MKP-5
• NCBI Protein Information: dual specificity protein phosphatase 10
• UniProt Protein Name: Dual specificity protein phosphatase 10
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP10
• UniProt Synonym Gene Names: MKP5; MAP kinase phosphatase 5; MKP-5
• UniProt Entry Name: DUS10_HUMAN

NCBI Description

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

MKP-5: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus

Molecular Function: MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of oligodendrocyte differentiation; negative regulation of JNK cascade; negative regulation of JNK activity; regulation of adaptive immune response; JNK cascade; oligodendrocyte differentiation; response to stress; response to lipopolysaccharide; protein amino acid dephosphorylation; inactivation of MAPK activity