Rabbit anti-Human DUSP7 Polyclonal Antibody | anti-DUSP7 antibody

DUSP7, NT (Dual Specificity Protein Phosphatase 7, Dual Specificity Protein Phosphatase PYST2, PYST2)

Cat. Num. AAA629287

• Gene Names: DUSP7; MKPX; PYST2; DKFZp586F2224
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DUSP7; Polyclonal Antibody; NT (Dual Specificity Protein Phosphatase 7; Dual Specificity Protein Phosphatase PYST2; PYST2); Anti -DUSP7; anti-DUSP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DUSP7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-DUSP7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human DUSP7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DUSP7 (arrow) using MBS629287. 293 cell lysates (2ug/lane) either Lane 1: nontransfected Lane 2: transiently transfected with the DUSP7 gene (Origene Technologies).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast cancer tissue using MBS629287 followed by peroxidase conjugation of the secondary antibody and AEC staining. This data demonstrates the use of MBS629287 for immunohistochemistry.)

Related Product Information for anti-DUSP7 antibody

DUSP7 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP)kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.

Product Categories/Family for anti-DUSP7 antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 238231453
• NCBI GeneID: 1849
• NCBI Accession #: NP_001938.2
• NCBI GenBank Nucleotide #: NM_001947.3
• UniProt Accession #: Q16829; Q2M3J7; Q8NFJ0
• Molecular Weight: 44,957 Da
• NCBI Official Full Name: dual specificity protein phosphatase 7
• NCBI Official Synonym Full Names: dual specificity phosphatase 7
• NCBI Official Symbol: DUSP7
• NCBI Official Synonym Symbols: MKPX; PYST2; DKFZp586F2224
• NCBI Protein Information: dual specificity protein phosphatase 7; OTTHUMP00000212488; dual-specificity phosphatase-7; dual specificity protein phosphatase PYST2
• UniProt Protein Name: Dual specificity protein phosphatase 7
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP7
• UniProt Synonym Gene Names: PYST2
• UniProt Entry Name: DUS7_HUMAN

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP7 belongs to a class of DUSPs, designated MKPs, that dephosphorylate MAPK (mitogen-activated protein kinase) proteins ERK (see MIM 601795), JNK (see MIM 601158), and p38 (see MIM 600289) with specificity distinct from that of individual MKP proteins. MKPs contain a highly conserved C-terminal catalytic domain and an N-terminal Cdc25 (see MIM 116947)-like (CH2) domain. MAPK activation cascades mediate various physiologic processes, including cellular proliferation, apoptosis, differentiation, and stress responses (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM]

Uniprot Description

DUSP7: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). High levels observed in the active state of AML and ALL diseases and in other types of cancer. 2 isoforms are produced by use of alternative promoters.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of MAP kinase activity; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor signaling pathway; innate immune response; toll-like receptor 3 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; inactivation of MAPK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway