Rabbit anti-Human, Mouse DTL Polyclonal Antibody | anti-DTL antibody

DTL, ID (DTL, CDT2, CDW1, DCAF2, L2DTL, RAMP, Denticleless protein homolog, DDB1-and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein) (Azide free) (HRP)

Cat. Num. AAA6293624

• Gene Names: DTL; CDT2; RAMP; DCAF2; L2DTL
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DTL; Polyclonal Antibody; ID (DTL; CDT2; CDW1; DCAF2; L2DTL; RAMP; Denticleless protein homolog; DDB1-and CUL4-associated factor 2; Lethal(2) denticleless protein homolog; Retinoic acid-regulated nuclear matrix-associated protein) (Azide free) (HRP); Retinoic acid-regulated nuclear matrix-associated protein; anti-DTL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DTL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DTL Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the DTL antibody detected the DTL protein (arrow).)

Flow Cytometry (FC/FACS)

(DTL Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DTL antibody

Antibodies; CD Markers

References

Centore, R.C., et al. Mol. Cell 40(1):22-33(2010). Abbas, T., et al. Mol. Cell 40(1):9-21(2010). Song, B., et al. Mol. Cancer 9, 96 (2010): Li, J., et al. Br. J. Cancer 101(4):691-698(2009). Abbas, T., et al. Genes Dev. 22(18):2496-2506(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 188219762
• NCBI GeneID: 51514
• NCBI Accession #: NP_057532.2
• NCBI GenBank Nucleotide #: NM_016448.2
• UniProt Accession #: Q9NZJ0; Q5VT77; Q96SN0; Q9NW03; Q9NW34; Q9NWM5; A8K8H8; D3DT98
• Molecular Weight: 79,468 Da
• NCBI Official Full Name: denticleless protein homolog
• NCBI Official Synonym Full Names: denticleless E3 ubiquitin protein ligase homolog (Drosophila)
• NCBI Official Symbol: DTL
• NCBI Official Synonym Symbols: CDT2; RAMP; DCAF2; L2DTL
• NCBI Protein Information: denticleless protein homolog; denticleless homolog; DDB1 and CUL4 associated factor 2; DDB1- and CUL4-associated factor 2; lethal(2) denticleless protein homolog; RA regulated nuclear matrix associated protein; RA-regulated nuclear matrix-associated prote
• UniProt Protein Name: Denticleless protein homolog
• Protein Family: Denticleless protein
• UniProt Gene Name: DTL
• UniProt Synonym Gene Names: CDT2; CDW1; DCAF2; L2DTL; RAMP
• UniProt Entry Name: DTL_HUMAN

Uniprot Description

RAMP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. Belongs to the WD repeat cdt2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; centrosome; nuclear membrane; intracellular membrane-bound organelle; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: protein monoubiquitination; ubiquitin-dependent protein catabolic process; bypass DNA synthesis; protein polyubiquitination; regulation of cell cycle; G2/M transition DNA damage checkpoint; DNA replication; response to DNA damage stimulus; response to UV