Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Macrophage Migration Inhibitory Factor (MIF) Standard Curve.)
Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit | MIF elisa kit
Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Macrophage Migration Inhibitory Factor (MIF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Macrophage Migration Inhibitory Factor (MIF) Standard Curve.)
Background: MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
NCBI and Uniprot Product Information
Uniprot Description
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.
Protein type: Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Apoptosis; Cell development/differentiation; Cytokine; EC 5.3.2.1; EC 5.3.3.12; Isomerase
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; nucleus; vesicle
Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein complex binding; receptor binding
Biological Process: brain renin-angiotensin system; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; drinking behavior; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive regulation of acute inflammatory response; positive regulation of axon regeneration; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycolysis; positive regulation of immune response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of potassium ion transport; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell migration; prostaglandin biosynthetic process; regulation of cell proliferation; spermatogenesis
Research Articles on MIF
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Product Notes
The Mouse MIF mif (Catalog #AAA9717633) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717633 ELISA Kit recognizes Mouse MIF. It is sometimes possible for the material contained within the vial of "Macrophage Migration Inhibitory Factor (MIF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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