Goat DSP / Desmoplakin Polyclonal Antibody | anti-DSP antibody

Goat Polyclonal to Human DSP / Desmoplakin

Cat. Num. AAA246767

• Gene Names: DSP; DP; DPI; DPII; DCWHKTA
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DSP / Desmoplakin; Polyclonal Antibody; Goat Polyclonal to Human DSP / Desmoplakin; Anti-DSP / Desmoplakin Antibody (Internal) IHC-plus; DSP; Apeg-1; Desmoplakin II; DP; Desmoplakin I; DPI; Desmoplakin (DPI; DPII); KPPS2; PPKS2; Desmoplakin; DPII; Human DSP; anti-DSP antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Mouse, Rat, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human Desmoplakin 1. This antibody is expected to recognize reported isoform I (NP_004406.2) only.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 2871

• Applicable Applications for anti-DSP antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:4000), IHC-P (3.75 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QESVKQAHLRNEH from an internal region of human DSP / Desmoplakin (NP_004406.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: DSP / Desmoplakin antibody was raised against synthetic peptide C-QESVKQAHLRNEH from an internal region of human DSP / Desmoplakin (NP_004406.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Monkey, Mouse, Rat, Hamster, Elephant, Dog, Horse, Rabbit, Opossum, Platypus (92%); Panda, Bat, Turkey, Chicken (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-DSP / Desmoplakin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Related Product Information for anti-DSP antibody

Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments.

NCBI and Uniprot Product Information

• NCBI GI #: 58530840
• NCBI GeneID: 1832
• NCBI Accession #: NP_004406.2
• NCBI GenBank Nucleotide #: NM_004415.2
• UniProt Accession #: P15924; O75993; Q14189; Q9UHN4; B2RTT2; D7RX09
• Molecular Weight: 278,916 Da
• NCBI Official Full Name: desmoplakin isoform I
• NCBI Official Synonym Full Names: desmoplakin
• NCBI Official Symbol: DSP
• NCBI Official Synonym Symbols: DP; DPI; DPII; DCWHKTA
• NCBI Protein Information: desmoplakin; desmoplakin I; desmoplakin II; 250/210 kDa paraneoplastic pemphigus antigen
• UniProt Protein Name: Desmoplakin
• Protein Family: Desmoplakin
• UniProt Gene Name: DSP
• UniProt Synonym Gene Names: DP
• UniProt Entry Name: DESP_HUMAN

NCBI Description

Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008]

Uniprot Description

Desmoplakin: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. Belongs to the plakin or cytolinker family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: desmosome; mitochondrion; basolateral plasma membrane; plasma membrane; cornified envelope; intermediate filament; fascia adherens; nucleus

Molecular Function: protein binding, bridging; protein binding; protein kinase C binding; structural constituent of cytoskeleton; cell adhesion molecule binding; structural molecule activity

Biological Process: keratinocyte differentiation; cell-cell adhesion; epidermis development; wound healing; apoptosis; intermediate filament organization; peptide cross-linking; cell structure disassembly during apoptosis

Disease: Cardiomyopathy, Dilated, With Woolly Hair And Keratoderma; Arrhythmogenic Right Ventricular Dysplasia, Familial, 8; Keratosis Palmoplantaris Striata Ii; Cardiomyopathy, Dilated, With Woolly Hair, Keratoderma, And Tooth Agenesis; Skin Fragility-woolly Hair Syndrome; Epidermolysis Bullosa, Lethal Acantholytic