Rabbit DOPAMINE TRANSPORTER Polyclonal Antibody | anti-SLC6A3 antibody

RABBIT ANTI HUMAN DOPAMINE TRANSPORTER (C-TERMINAL)

Cat. Num. AAA221820

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Reactivity: Monkey, Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: DOPAMINE TRANSPORTER; Polyclonal Antibody; RABBIT ANTI HUMAN DOPAMINE TRANSPORTER (C-TERMINAL); anti-SLC6A3 antibody

• Host: Rabbit
• Reactivity: Monkey, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the C-terminal region (CT) of dopamine transporter (DAT), also known as solute carrier family 6. ; ; DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine. ; ; The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 620

• Applicable Applications for anti-SLC6A3 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 88kDa in human caudate cell lysates.; Immunohistology - Frozen: Maximum Dilution: 1/1000; Western Blotting: Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Keyhole limpet haemocyanin conjugated synthetic peptide corresponding to the C-terminus region (CT) of human dopamine transporter.; Buffer Solution
• Antiserum Preparation: Antisera to human dopamine transporter (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-SLC6A3 antibody

MBS221820 recognises an epitope within the C-terminal region (CT) of dopamine transporter (DAT), also known as solute carrier family 6. DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine. The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.

NCBI and Uniprot Product Information

• NCBI GI #: 4507041
• NCBI GeneID: 6531
• NCBI Accession #: NP_001035.1
• NCBI GenBank Nucleotide #: NM_001044.4
• UniProt Accession #: Q01959; Q14996; A2RUN4
• Molecular Weight: 68,495 Da
• NCBI Official Full Name: sodium-dependent dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter; DA transporter; dopamine transporter 1; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• UniProt Protein Name: Sodium-dependent dopamine transporter
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN

NCBI Description

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Uniprot Description

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell soma; axon; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; protease binding; protein complex binding; monoamine transmembrane transporter activity; dopamine binding; protein N-terminus binding; dopamine:sodium symporter activity; drug binding; dopamine transmembrane transporter activity; receptor binding

Biological Process: lactation; response to drug; response to nicotine; dopamine transport; response to cAMP; prepulse inhibition; dopamine catabolic process; monoamine transport; regulation of dopamine metabolic process; sensory perception of smell; locomotory behavior; dopamine biosynthetic process; positive regulation of multicellular organism growth; response to cocaine; synaptic transmission; response to ethanol; neurotransmitter transport; adenohypophysis development; transmembrane transport; response to iron ion; aging; neurotransmitter biosynthetic process

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To