Rat Sodium-dependent dopamine transporter ELISA Kit | Slc6a3 elisa kit

Rat Sodium-dependent dopamine transporter ELISA Kit

Cat. Num. AAA760607

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Reactivity: Rat
• Synonyms: Sodium-dependent dopamine transporter; Rat Sodium-dependent dopamine transporter ELISA Kit; Slc6a3/DAT1/SLC6A3/VNTR/DA transporter/DATDAT1Solute carrier family 6 member 3/sodium-dependent dopamine transporter/solute carrier family 6 (neurotransmitter transporter; dopamine); member 3; Slc6a3 elisa kit

• Reactivity: Rat
• Sequence Length: 620

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slc6a3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Slc6a3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Slc6a3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Slc6a3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Slc6a3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507041
• NCBI GeneID: 6531
• NCBI Accession #: NP_001035.1
• NCBI GenBank Nucleotide #: NM_001044.4
• Molecular Weight: 68,495 Da
• NCBI Official Full Name: sodium-dependent dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter
• UniProt Protein Name: Sodium-dependent dopamine transporter
• Protein Family: Sodium-dependent dopamine transporter
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN

NCBI Description

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Uniprot Description

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: axon; cell soma; cell surface; cytoplasm; flotillin complex; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: dopamine binding; dopamine transmembrane transporter activity; dopamine:sodium symporter activity; drug binding; monoamine transmembrane transporter activity; protease binding; protein binding; protein complex binding; protein N-terminus binding; protein phosphatase 2A binding; receptor binding

Biological Process: adenohypophysis development; aging; dopamine biosynthetic process; dopamine catabolic process; dopamine transport; dopamine uptake; lactation; locomotory behavior; monoamine transport; neurotransmitter biosynthetic process; positive regulation of multicellular organism growth; prepulse inhibition; regulation of dopamine metabolic process; response to cAMP; response to cocaine; response to drug; response to ethanol; response to iron ion; response to nicotine; sensory perception of smell; synaptic transmission; transmembrane transport

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To