Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissues (Fig. 1 and Fig. 2) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilutionAnti-c-ErbB2/c-Neu, clone 3B5. Reactivity was detected using an Anti-Mouse IgG secondary antibody and HRP-DAB. Positive cell ular membrane and cytoplasmic staining was observed in human breast cancer tissue (Fig. 1). (Fig. 2 illustrates the use of a negative control.))
Mouse anti-Human, Mouse ErbB2 Monoclonal Antibody | anti-ErbB2 antibody
ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, CD340, p185erbB2, HER2, MLN19, NEU, NGL) (HRP)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissues (Fig. 1 and Fig. 2) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilutionAnti-c-ErbB2/c-Neu, clone 3B5. Reactivity was detected using an Anti-Mouse IgG secondary antibody and HRP-DAB. Positive cell ular membrane and cytoplasmic staining was observed in human breast cancer tissue (Fig. 1). (Fig. 2 illustrates the use of a negative control.))
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human breast cancer tissues (Fig. 1 and Fig. 2) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:1,000 dilutionAnti-c-ErbB2/c-Neu, clone 3B5. Reactivity was detected using an Anti-Mouse IgG secondary antibody and HRP-DAB. Positive cell ular membrane and cytoplasmic staining was observed in human breast cancer tissue (Fig. 1). (Fig. 2 illustrates the use of a negative control.))
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. SK-BR-3 cell lysate was probed Anti-c-ErbB2/c-Neu, clone 3B5 (0.5ug/ml). Proteins were visualized using a Goat Anti-Mouse IgG (H&L) secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ErbB2/c-Neu (~185kD). Uniprot describes four isoforms at ~138kD (isoform 1), ~63kD (isoform 2), ~71kD (isoform 3), and ~137kD (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27kD and ~38kD in some cell lysates.)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. SK-BR-3 cell lysate was probed Anti-c-ErbB2/c-Neu, clone 3B5 (0.5ug/ml). Proteins were visualized using a Goat Anti-Mouse IgG (H&L) secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates ErbB2/c-Neu (~185kD). Uniprot describes four isoforms at ~138kD (isoform 1), ~63kD (isoform 2), ~71kD (isoform 3), and ~137kD (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27kD and ~38kD in some cell lysates.)
NCBI and Uniprot Product Information
Uniprot Description
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.
Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Oncoprotein; TK group; EGFR family
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex
Molecular Function: protein dimerization activity; protein C-terminus binding; identical protein binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; ATP binding
Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion; wound healing; nerve growth factor receptor signaling pathway; positive regulation of translation; heart development; protein amino acid autophosphorylation; motor axon guidance; signal transduction; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; epidermal growth factor receptor signaling pathway; negative regulation of immature T cell proliferation in the thymus; regulation of microtubule-based process; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; regulation of angiogenesis; positive regulation of cell growth; peripheral nervous system development; cell proliferation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of protein amino acid phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
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Product Notes
The ErbB2 erbb2 (Catalog #AAA6244141) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, CD340, p185erbB2, HER2, MLN19, NEU, NGL) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the ErbB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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