Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using Smad9 antibody.)
Rabbit Smad9 Polyclonal Antibody | anti-Smad9 antibody
Smad9 Antibody
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Smad9; Polyclonal Antibody; Smad9 Antibody; MAD homolog 9; Madh6; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD; mothers against DPP homolog 9 (Drosophila); SMAD8A; SMAD8B; SMAD9_HUMAN; anti-Smad9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Smad9 antibody detects endogenous levels of total Smad9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-Smad9 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human Smad9
Subcellular Location
Cytoplasmic and Nuclear
Tissue Specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Predicted Cross Reactivity
Sheep, Chicken
Similarity
Sheep (100%), Chicken (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using Smad9 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using Smad9 antibody.)
Immunohistochemistry (IHC)
(IHC test Smad9 antibody in human skin tissue.)
Immunohistochemistry (IHC)
(IHC test Smad9 antibody in human skin tissue.)
Related Product Information for anti-Smad9 antibody
Description: SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.
Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Subunit Structure: Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit. Interacts with RANBP3L (PubMed:25755279).
Post-translational Modifications: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Similarity: Belongs to the dwarfin/SMAD family.
Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Subunit Structure: Interaction with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit. Interacts with RANBP3L (PubMed:25755279).
Post-translational Modifications: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Similarity: Belongs to the dwarfin/SMAD family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 52 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
mothers against decapentaplegic homolog 9 isoform a
NCBI Official Synonym Full Names
SMAD family member 9
NCBI Official Symbol
SMAD9
NCBI Official Synonym Symbols
PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
NCBI Protein Information
mothers against decapentaplegic homolog 9
UniProt Protein Name
Mothers against decapentaplegic homolog 9
Protein Family
UniProt Gene Name
SMAD9
UniProt Synonym Gene Names
; MAD homolog 9; Mothers against DPP homolog 9; SMAD 9; Smad9
NCBI Description
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
Uniprot Description
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Research Articles on Smad9
Similar Products
Product Notes
The Smad9 smad9 (Catalog #AAA9601287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smad9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Smad9 smad9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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