Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)
Rabbit anti-Human Dopamine Beta Hydroxylase Polyclonal Antibody | anti-DbH antibody
Polyclonal Antibody to Dopamine Beta Hydroxylase (DbH)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human HepG2 Cells; Lane2: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DbH, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DbH, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Breast cancer Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: DbH knockout Hela cell lysate; Predicted MW: 69kd Observed MW: 69kd Primary Ab: 5ug/ml Rabbit Anti-Human DbH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: DbH knockout Hela cell lysate; Predicted MW: 69kd Observed MW: 69kd Primary Ab: 5ug/ml Rabbit Anti-Human DbH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
Uniprot Description
DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
Protein type: Amino Acid Metabolism - tyrosine; Apoptosis; Cell cycle regulation; EC 1.14.17.1; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase
Chromosomal Location of Human Ortholog: 9q34.2
Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; intracellular membrane-bound organelle; membrane; secretory granule membrane
Molecular Function: catalytic activity; copper ion binding; dopamine beta-monooxygenase activity; L-ascorbic acid binding
Biological Process: behavioral response to ethanol; blood vessel remodeling; catecholamine biosynthetic process; cytokine production; dopamine catabolic process; fear response; glucose homeostasis; homoiothermy; leukocyte mediated immunity; leukocyte migration; locomotory behavior; maternal behavior; memory; norepinephrine biosynthetic process; octopamine biosynthetic process; positive regulation of vasoconstriction; regulation of cell proliferation; response to amphetamine; response to pain; synaptic transmission; visual learning
Disease: Dopamine Beta-hydroxylase Deficiency, Congenital
Research Articles on DbH
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Product Notes
The DbH dbh (Catalog #AAA2027051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Dopamine Beta Hydroxylase (DbH) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dopamine Beta Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DbH dbh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dopamine Beta Hydroxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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