Typical Testing Data/Standard Curve (for reference only)
Human Dopamine beta-hydroxylase ELISA Kit | DBH elisa kit
Human Dopamine beta-hydroxylase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine beta-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dopamine beta-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dopamine beta-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Dopamine beta-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
Uniprot Description
DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
Protein type: Amino Acid Metabolism - tyrosine; Cell cycle regulation; Apoptosis; EC 1.14.17.1; Oxidoreductase; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: cytoplasm; extracellular region; integral to membrane; membrane
Molecular Function: catalytic activity; copper ion binding; dopamine beta-monooxygenase activity; L-ascorbic acid binding
Biological Process: behavioral response to ethanol; blood vessel remodeling; catecholamine biosynthetic process; cytokine production; dopamine catabolic process; fear response; glucose homeostasis; homoiothermy; leukocyte mediated immunity; leukocyte migration; locomotory behavior; maternal behavior; memory; norepinephrine biosynthetic process; positive regulation of vasoconstriction; regulation of cell proliferation; response to amphetamine; response to pain; synaptic transmission; visual learning
Disease: Dopamine Beta-hydroxylase Deficiency, Congenital
Research Articles on DBH
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Product Notes
The Human DBH dbh (Catalog #AAA2885836) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885836 ELISA Kit recognizes Human DBH. It is sometimes possible for the material contained within the vial of "Dopamine beta-hydroxylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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