MVK recombinant protein

MVK, 1-396aa, Human, His tag, E Coli

Cat. Num. AAA205405

• Gene Names: MVK; MK; LRBP; MVLK; POROK3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MVK; 1-396aa; Human; His tag; E Coli; Mevalonate kinase; LRBP; MK; MVLK; MVK recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLSEVLL VSAPGKVILH GEHAVVHGKV ALAVSLNLRT FLRLQPHSNG KVDLSLPNIG IKRAWDVARL QSLDTSFLEQ GDVTTPTSEQ VEKLKEVAGL PDDCAVTERL AVLAFLYLYL SICRKQRALP SLDIVVWSEL PPGAGLGSSA AYSVCLAAAL LTVCEEIPNP LKDGDCVNRW TKEDLELINK WAFQGERMIH GNPSGVDNAV STWGGALRYH QGKISSLKRS PALQILLTNT KVPRNTRALV AGVRNRLLKF PEIVAPLLTS IDAISLECER VLGEMGEAPA PEQYLVLEEL IDMNQHHLNA LGVGHASLDQ LCQVTRARGL HSKLTGAGGG GCGITLLKPG LEQPEVEATK QALTSCGFDC LETSIGAPGV SIHSATSLDS RVQQALDGL
• Sequence Length: 396

• Applicable Applications for MVK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MVK recombinant protein

Mevalonate kinase, also known as MVK, belongs to the GHMP kinase family. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive,hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Recombinant human MVK protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MVK recombinant protein

Metabolism

References

Cuisset L., et al. (2001) Eur. J. Hum. Genet. 9:260-266; D'Osualdo A., et al. (2005) Eur. J. Hum. Genet. 13:314-320

NCBI and Uniprot Product Information

• NCBI GI #: 167001643
• NCBI GeneID: 4598
• NCBI Accession #: NP_001107657
• NCBI GenBank Nucleotide #: NM_001114185.2
• UniProt Accession #: Q03426
• Molecular Weight: 44.8 kDa (419aa) confirmed by MALDI-TOF
• NCBI Official Full Name: mevalonate kinase isoform a
• NCBI Official Synonym Full Names: mevalonate kinase
• NCBI Official Symbol: MVK
• NCBI Official Synonym Symbols: MK; LRBP; MVLK; POROK3
• NCBI Protein Information: mevalonate kinase
• UniProt Protein Name: Mevalonate kinase
• Protein Family: Mevalonate kinase
• UniProt Gene Name: MVK
• UniProt Synonym Gene Names: MK

NCBI Description

This gene encodes the peroxisomal enzyme mevalonate kinase. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive, hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

May be a regulatory site in cholesterol biosynthetic pathway.