Western Blot (WB)
(Host: RabbitTarget Name: DOC2BSample Tissue: Human HepG2 Whole Cell lysatesAntibody Dilution: 1ug/ml)
Rabbit anti-Human DOC2B Polyclonal Antibody | anti-DOC2B antibody
DOC2B Antibody - middle region
Western Blot (WB)
(Host: RabbitTarget Name: DOC2BSample Tissue: Human HepG2 Whole Cell lysatesAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: DOC2BSample Tissue: Human HepG2 Whole Cell lysatesAntibody Dilution: 1ug/ml)
NCBI and Uniprot Product Information
NCBI Description
There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes
By similarity. Ref.2
Subunit structure: Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion. May interact with UNC13A; the interaction mediates targeting to the plasma membrane
By similarity. Interacts with cytoplasmic dynein light chain DYNLT1. Ref.2
Subcellular location: Cytoplasm. Cytoplasmic granule
By similarity. Cell membrane; Peripheral membrane protein
By similarity. Note: Translocates to the plasma membrane in a calcium-dependent manner
By similarity.
Tissue specificity: Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level). Ref.1 Ref.3
Domain: C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes
By similarity.
Sequence similarities: Contains 2 C2 domains.
Research Articles on DOC2B
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Product Notes
The DOC2B doc2b (Catalog #AAA3221263) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOC2B Antibody - middle region reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOC2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DOC2B doc2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: KTYLRPDVDK KSKHKTAVKK KTLNPEFNEE FCYEIKHGDL AKKSLEVTVW. It is sometimes possible for the material contained within the vial of "DOC2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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