Rabbit anti-Human DNMT3L Polyclonal Antibody | anti-DNMT3L antibody

Anti-Human DNMT3L Antibody

Cat. Num. AAA4156747

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: DNMT3L; Polyclonal Antibody; Anti-Human DNMT3L Antibody; Human DNMT3L; anti-DNMT3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 386

• Applicable Applications for anti-DNMT3L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 152-164 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-DNMT3L antibody

DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

NCBI and Uniprot Product Information

• NCBI GI #: 28872780
• NCBI GeneID: 29947
• NCBI GenBank Nucleotide #: NP_787063.1
• UniProt Accession #: Q9UJW3
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 2
• NCBI Official Synonym Full Names: DNA methyltransferase 3 like
• NCBI Official Symbol: DNMT3L
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: DNMT3L
• UniProt Entry Name: DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]