Rabbit GW182/TNRC6A Polyclonal Antibody | anti-TNRC6A antibody

Anti-GW182/TNRC6A Antibody, Rabbit Polyclonal

Cat. Num. AAA8101265

• Gene Names: TNRC6A; GW1; GW182; TNRC6; CAGH26
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GW182/TNRC6A; Polyclonal Antibody; Anti-GW182/TNRC6A Antibody; Rabbit Polyclonal; TNRC6A Antibody; Rabbit PAb; Antigen Affinity Purified; trinucleotide repeat containing 6A; Anti-CAGH26 Antibody; Anti-GW1 Antibody; Anti-GW182 Antibody; Anti-TNRC6 Antibody; anti-TNRC6A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GW182/TNRC6A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TNRC6A antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:500
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human TNRC6A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TNRC6A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human TNRC6A in human breast carcinoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-TNRC6A antibody

The GW182/TNRC6 family of proteins are central scaffolds that link microRNA-associated Argonaute proteins to the cytoplasmic decay machinery for targeted mRNA degradation processes. Trinucleotide repeat-containing gene 6A protein (TNRC6A) is an essential protein for microRNA-mediated gene silencing. TNRC6A functions in the cytoplasm as a platform protein interacting with Argonaute protein, on which microRNA is loaded for RNA silencing, and decapping enzymes or deadenylation protein complexes to induce mRNA degradation. TNRC6A, is a nuclear-cytoplasmic shuttling protein, and its subcellular localization is regulated by its own nuclear localization signal and nuclear export signal.

NCBI and Uniprot Product Information

• NCBI GI #: 116805348
• NCBI GeneID: 27327
• NCBI Accession #: NP_055309.2
• NCBI GenBank Nucleotide #: NM_014494.3
• UniProt Accession #: Q8NDV7; O15408; Q658L5; Q6NVB5; Q8NEZ0; Q8TBT8; Q8TCR0; Q9NV59; Q9P268; C9JAR8
• Molecular Weight: 205,151 Da
• NCBI Official Full Name: trinucleotide repeat-containing gene 6A protein isoform 1
• NCBI Official Synonym Full Names: trinucleotide repeat containing 6A
• NCBI Official Symbol: TNRC6A
• NCBI Official Synonym Symbols: GW1; GW182; TNRC6; CAGH26
• NCBI Protein Information: trinucleotide repeat-containing gene 6A protein
• UniProt Protein Name: Trinucleotide repeat-containing gene 6A protein
• Protein Family: Trinucleotide repeat-containing gene 6A protein
• UniProt Gene Name: TNRC6A
• UniProt Synonym Gene Names: CAGH26; KIAA1460; TNRC6; Protein GW1

NCBI Description

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.