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Western Blot (WB) (Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)

Rabbit anti-Human Dnmt3L Polyclonal Antibody | anti-DNMT3L antibody

Dnmt3L, CT (DNMT3L, DNA (cytosine-5)-methyltransferase 3-like)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Dnmt3L; Polyclonal Antibody; CT (DNMT3L; DNA (cytosine-5)-methyltransferase 3-like); Anti -Dnmt3L; anti-DNMT3L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-DNMT3L antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot1:100-500
Immunohistochemistry: 1:50-100
Immunogen
Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388~417 amino acids from the C-terminal region of human Dnmt3L.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)

Western Blot (WB) (Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-DNMT3L antibody
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.
Product Categories/Family for anti-DNMT3L antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,583 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3-like isoform 2
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3-like
NCBI Official Symbol
DNMT3L
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3-like
UniProt Gene Name
DNMT3L
UniProt Entry Name
DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity

Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine; regulation of gene expression, epigenetic

Research Articles on DNMT3L

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Product Notes

The DNMT3L dnmt3l (Catalog #AAA6008911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dnmt3L, CT (DNMT3L, DNA (cytosine-5)-methyltransferase 3-like) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dnmt3L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the DNMT3L dnmt3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dnmt3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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