Rabbit anti-Human DNMT3L Polyclonal Antibody | anti-DNMT3L antibody

Rabbit Polyclonal to Human DNMT3L

Cat. Num. AAA241018

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Protein G Purified
• Synonyms: DNMT3L; Polyclonal Antibody; Rabbit Polyclonal to Human DNMT3L; Anti-DNMT3L Antibody (aa322-337) IHC-plus; Human DNMT3L; anti-DNMT3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 322 to 337 of human DNMT3L
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 387

• Applicable Applications for anti-DNMT3L antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:000 - 1:1000), IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human DNMT3L.
• Immunogen Type: Synthetic peptide
• Immunogen: DNMT3L antibody was raised against synthetic peptide from human DNMT3L.
• Antigen Modification: aa322-337
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-DNMT3L antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-DNMT3L antibody

DNA cytosine-5-methyltransferase-3 proteins are involved in de novo methylation of CpG islands. Mutations in the DNMT3B gene cause the ICF immunodeficiency syndrome. DNA methyltransferases and regulatory factors are likely to be involved in the establishment of maternal imprints in growing diplotene oocytes and paternal imprints in perinatal prospermatogonia. Imprinting is essential for viable development.

Product Categories/Family for anti-DNMT3L antibody

Family: C5-methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 28872778
• NCBI GeneID: 29947
• NCBI Accession #: NP_037501.2
• NCBI GenBank Nucleotide #: NM_013369.3
• UniProt Accession #: Q9UJW3; Q9BUJ4; E9PB42
• Molecular Weight: 43,670 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 1
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3-like
• NCBI Official Symbol: DNMT3L
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: DNMT3L
• UniProt Entry Name: DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity

Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; DNA methylation on cytosine