Western Blot (WB)
(Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)
Rabbit anti-Human Dnmt3L Polyclonal Antibody | anti-Dnmt3L antibody
Dnmt3L, CT (DNMT3L, DNA (cytosine-5)-methyltransferase 3-like) (AP)
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Dnmt3L; Polyclonal Antibody; CT (DNMT3L; DNA (cytosine-5)-methyltransferase 3-like) (AP); DNMT3L; DNA (cytosine-5)-methyltransferase 3-like; anti-Dnmt3L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Dnmt3L antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 388~417 amino acids from the C-terminal region of human Dnmt3L.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)
Western Blot (WB)
(Dnmt3L-R322 western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNMT3L antibody detected the DNMT3L protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-Dnmt3L antibody
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.
Product Categories/Family for anti-Dnmt3L antibody
References
Chedin, F., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16916-16921 (2002). Kierszenbaum, A.L., Mol. Reprod. Dev. 63(3):269-272 (2002). Deplus, R., et al., Nucleic Acids Res. 30(17):3831-3838 (2002). Hata, K., et al., Development 129(8):1983-1993 (2002). Burgers, W.A., et al., Trends Genet. 18(6):275-277 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
386
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3-like
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3-like
NCBI Official Symbol
DNMT3L
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3-like
Protein Family
UniProt Gene Name
DNMT3L
UniProt Entry Name
DNM3L_HUMAN
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Product Notes
The Dnmt3L dnmt3l (Catalog #AAA6293125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dnmt3L, CT (DNMT3L, DNA (cytosine-5)-methyltransferase 3-like) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dnmt3L can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Dnmt3L dnmt3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dnmt3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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