Rat CD160 Monoclonal Antibody | anti-CD160 antibody

CD160 antibody (PE)

Cat. Num. AAA832170

• Gene Names: CD160; NK1; BY55; NK28
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD160; Monoclonal Antibody; CD160 antibody (PE); Monoclonal CD160; Anti-CD160; NK1; CD-160; CD 160; NK28; BY55; anti-CD160 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a kappa
• Clone Number: CNX46-3
• Specificity: Human
• Form/Format: Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
• Concentration: 200 ug/ml (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-CD160 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC: <= 0.125 ug/test; ; A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Biological Significance: CD160 is a glycosylphosphatidylinositol -anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all iIELs, NKT cells, most TCRgamma delta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160. In human, CD160 mAb cross-linking triggers TNF alpha, IFNgamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.
• Tag/Conjugate: PE
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Staining of C57BL/6 splenocytes with CD3 antibody (FITC), NK1.1 antibody (APC), and 0.5 ug of CD160 antibody (PE). The histogram (right) demonstrates staining of CD160 on NK1.1+CD3- cells (red histogram) and NK1.1+CD3+ cells (black histogram), as gated in the dot plot (left).)

Related Product Information for anti-CD160 antibody

Rat monoclonal CD160 antibody (PE)

Product Categories/Family for anti-CD160 antibody

Immunology; Conjugated Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5901910
• NCBI GeneID: 11126
• NCBI Accession #: NP_008984.1
• NCBI GenBank Nucleotide #: NM_007053.3
• UniProt Accession #: O95971
• Molecular Weight: 19,810 Da
• NCBI Official Full Name: CD160 antigen
• NCBI Official Synonym Full Names: CD160 molecule
• NCBI Official Symbol: CD160
• NCBI Official Synonym Symbols: NK1; BY55; NK28
• NCBI Protein Information: CD160 antigen
• UniProt Protein Name: CD160 antigen
• Protein Family: CD160 antigen
• UniProt Gene Name: CD160
• UniProt Synonym Gene Names: BY55
• UniProt Entry Name: BY55_HUMAN

NCBI Description

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor showing broad specificity for both classical and non-classical MHC class I molecules.