Rabbit anti-Human ARID3B Polyclonal Antibody | anti-ARID3B antibody

Anti-ARID3B Antibody, Rabbit Polyclonal

Cat. Num. AAA8100271

• Gene Names: ARID3B; BDP; DRIL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ARID3B; Polyclonal Antibody; Anti-ARID3B Antibody; Rabbit Polyclonal; ARID3B Antibody; Rabbit PAb; Antigen Affinity Purified; AT-rich interaction domain 3B; Anti-BDP Antibody; Anti-DRIL2 Antibody; anti-ARID3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ARID3B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ARID3B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the human ARID3B
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the human ARID3B, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ARID3B rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-ARID3B antibody

ARID3B, a member of the AT-rich interaction domain (ARID) family of proteins, plays an essential role in the survival of neural crest during embryogenesis. ARID3B seems to play a key role in the malignant transformation of neuroblastoma and may serve not only as a marker of malignancy but also as a potential target for cancer therapy of stage IV neuroblastoma for which there is currently no effective treatment available. ARID3B is a DNA binding protein that is overexpressed in neuroblastoma and ovarian cancer. ARID3B has different patterns in normal tissues translate into different roles for ARID3B in carcinomas. ARID3B (AT-rich interaction domain 3) is a member of the family of ARID proteins, which constitutes evolutionarily conserved transcription factors implicated in normal development, differentiation, cell cycle regulation and chromatin remodeling. In addition, ARID3B has been linked to cellular immortalization, epithelial-mesenchymal transition (EMT) and tumorigenesis. Given the emerging role of ARID3B in tumor development, we examined its expression in primary patient-derived breast cancer samples and breast cancer-derived cell lines. ARID3B regulation of direct target genes in the Wnt pathway promotes adhesion of ovarian cancer cells. ARID3A and ARID3B are transcriptional targets of p53, ARID3B play a key role in the expression of pro-apoptotic p53-target genes and apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 5453638
• NCBI GeneID: 10620
• NCBI Accession #: NP_006456.1
• NCBI GenBank Nucleotide #: NM_006465.2
• UniProt Accession #: Q8IVW6; O95443; Q59HC9; Q6P9C9
• Molecular Weight: 60,566 Da
• NCBI Official Full Name: AT-rich interactive domain-containing protein 3B
• NCBI Official Synonym Full Names: AT rich interactive domain 3B (BRIGHT-like)
• NCBI Official Symbol: ARID3B
• NCBI Official Synonym Symbols: BDP; DRIL2
• NCBI Protein Information: AT-rich interactive domain-containing protein 3B; bright-like protein; bright and dead ringer protein; ARID domain-containing protein 3B; AT rich interactive domain 3B (BRIGHT- like)
• UniProt Protein Name: AT-rich interactive domain-containing protein 3B
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID3B
• UniProt Synonym Gene Names: BDP; DRIL2; ARID domain-containing protein 3B
• UniProt Entry Name: ARI3B_HUMAN

NCBI Description

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. The encoded protein is homologous with two proteins that bind to the retinoblastoma gene product, and also with the mouse Bright and Drosophila dead ringer proteins. A pseudogene on chromosome 1p31 exists for this gene. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification. [provided by RefSeq, Jul 2008]

Uniprot Description

ARID3B: Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter