Rabbit anti-Human Dnmt1 Polyclonal Antibody | anti-Dnmt1 antibody

Dnmt1(Phospho-Ser714) antibody

Cat. Num. AAA9406081

• Gene Names: DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Dnmt1; Polyclonal Antibody; Dnmt1(Phospho-Ser714) antibody; AIM; DNMT; MCMT; CXXC9; HSN1E; FLJ16293; MGC104992; DNMT1; anti-Dnmt1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Dnmt1 only when phosphorylated at serine 714.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1632

• Applicable Applications for anti-Dnmt1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Peptide
• Immunogen Description: A phospho specific peptide corresponding to residues surrounding S714 of human Dnmt1.
• Modification: Phospho-Ser714
• Target Name: Dnmt1
• Preparation and Storage: Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using Phospho-Dnmt1-pS714 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-Dnmt1 antibody

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).

Product Categories/Family for anti-Dnmt1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 195927037
• NCBI GeneID: 1786
• NCBI Accession #: NP_001124295.1
• NCBI GenBank Nucleotide #: NM_001130823.1
• UniProt Accession #: P26358; Q9UHG5; Q9ULA2; Q9UMZ6; A0AV63; B7ZLW6
• Molecular Weight: 144,465 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1 isoform a
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 1
• NCBI Official Symbol: DNMT1
• NCBI Official Synonym Symbols: AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: DNMT1
• UniProt Synonym Gene Names: AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
• UniProt Entry Name: DNMT1_HUMAN

NCBI Description

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding

Biological Process: negative regulation of histone H3-K9 methylation; maintenance of DNA methylation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; positive regulation of histone H3-K4 methylation; gene silencing; DNA methylation on cytosine; regulation of gene expression, epigenetic; regulation of cell proliferation

Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie