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Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DNAJB1 antibody.)
Rabbit DNAJB1 Polyclonal Antibody | anti-DNAJB1 antibody
DNAJB1 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock Protein 40, Hdj1, Sis1, HSPF1, Hsp40, RSPH16B) (HRP)
Gene Names
DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
DNAJB1; Polyclonal Antibody; DNAJB1 (DnaJ Homolog Subfamily B Member 1; Heat Shock 40kD Protein 1; Heat Shock Protein 40; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B) (HRP); anti-DNAJB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes DNAJB1. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-DNAJB1 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human DNAJB1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DNAJB1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using DNAJB1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using DNAJB1 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using DNAJB1 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB1 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney cancer using DNAJB1 antibody at dilution of 1:200 (200x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung using DNAJB1 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung using DNAJB1 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse spleen using DNAJB1 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse spleen using DNAJB1 antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using DNAJB1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using DNAJB1 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-DNAJB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,044 Da
NCBI Official Full Name
dnaJ homolog subfamily B member 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily B, member 1
NCBI Official Symbol
DNAJB1
NCBI Official Synonym Symbols
Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
NCBI Protein Information
dnaJ homolog subfamily B member 1; hDj-1; human DnaJ protein 1; heat shock protein 40; dnaJ protein homolog 1; heat shock 40kD protein 1; radial spoke 16 homolog B; heat shock 40 kDa protein 1; DnaJ (Hsp40) homolog, subfmaily B, member 1
UniProt Protein Name
DnaJ homolog subfamily B member 1
Protein Family
UniProt Gene Name
DNAJB1
UniProt Synonym Gene Names
DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1
UniProt Entry Name
DNJB1_HUMAN
Uniprot Description
Function: Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Subunit structure: Interacts with DNAJC3. Interacts with SRPK1. Ref.6 Ref.7
Subcellular location: Cytoplasm. Nucleus. Nucleus › nucleolus. Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Ref.5
Induction: By heat shock. Ref.5
Sequence similarities: Contains 1 J domain.
Sequence caution: The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.
Research Articles on DNAJB1
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Product Notes
The DNAJB1 dnajb1 (Catalog #AAA6414323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJB1 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock Protein 40, Hdj1, Sis1, HSPF1, Hsp40, RSPH16B) (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DNAJB1 dnajb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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