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Western Blot (WB) (Western blot analysis of lysates from HepG2, SK-BR-3, Raji cell line, mouse lung tissue lysate and rat PC-12 cell line (from left to right), using DNAJA1 Antibody (C-term). MBS9205999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Rabbit DNAJA1 Polyclonal Antibody | anti-DNAJA1 antibody

DNAJA1 Antibody (C-term)

Gene Names
DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DNAJA1; Polyclonal Antibody; DNAJA1 Antibody (C-term); DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2; DNAJ2; HDJ2; HSJ2; HSPF4; anti-DNAJA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DNAJA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 379-414 amino acids from the C-terminal region of human DNAJA1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
397
Applicable Applications for anti-DNAJA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from HepG2, SK-BR-3, Raji cell line, mouse lung tissue lysate and rat PC-12 cell line (from left to right), using DNAJA1 Antibody (C-term). MBS9205999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from HepG2, SK-BR-3, Raji cell line, mouse lung tissue lysate and rat PC-12 cell line (from left to right), using DNAJA1 Antibody (C-term). MBS9205999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-DNAJA1 antibody
Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
Product Categories/Family for anti-DNAJA1 antibody
References
Oh S.,et al.Biochim. Biophys. Acta 1174:114-116(1993).
Chellaiah A.,et al.Biochim. Biophys. Acta 1174:111-113(1993).
Hu Y.,et al.Int. J. Androl. 27:343-349(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44868
NCBI Official Full Name
dnaJ homolog subfamily A member 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily A, member 1
NCBI Official Symbol
DNAJA1
NCBI Official Synonym Symbols
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
NCBI Protein Information
dnaJ homolog subfamily A member 1
UniProt Protein Name
DnaJ homolog subfamily A member 1
Protein Family
UniProt Gene Name
DNAJA1
UniProt Synonym Gene Names
DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2
UniProt Entry Name
DNJA1_HUMAN

Uniprot Description

DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

Protein type: Nuclear receptor co-regulator; Chaperone

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: mitochondrion; membrane; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; metal ion binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; ATP binding

Biological Process: regulation of protein transport; negative regulation of JNK activity; sperm motility; protein folding; response to heat; positive regulation of apoptosis; androgen receptor signaling pathway; negative regulation of protein ubiquitination; DNA damage response, detection of DNA damage; protein refolding; spermatogenesis; response to unfolded protein; negative regulation of apoptosis

Research Articles on DNAJA1

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Product Notes

The DNAJA1 dnaja1 (Catalog #AAA9205999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJA1 Antibody (C-term) reacts with Human, mouse, rat (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the DNAJA1 dnaja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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