Typical Testing Data/Standard Curve
Human C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit | C5AR1 elisa kit
Human C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||C5AR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C5AR1 antibody and an C5AR1-HRP conjugate. The assay sample and buffer are incubated together with C5AR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C5AR1 concentration since C5AR1 from samples and C5AR1-HRP conjugate compete for the anti-C5AR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by C5AR1 from the sample, fewer sites are left to bind C5AR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5AR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane
Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity
Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; activation of MAPK activity; apoptosis; response to lipopolysaccharide; cell proliferation in hindbrain; chemotaxis; signal transduction; mRNA transcription from RNA polymerase II promoter; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation
Research Articles on C5AR1
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Product Notes
The Human C5AR1 c5ar1 (Catalog #AAA7243363) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7243363 ELISA Kit recognizes Human C5AR1. It is sometimes possible for the material contained within the vial of "C5a anaphylatoxin chemotactic receptor (C5AR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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