Rabbit anti-Human DNA Methyltransferase 3L Polyclonal Antibody
DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5' Methyltransferase 3 Like Protein)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
DNA Methyltransferase 3L; Polyclonal Antibody; DNA Methyltransferase 3L (Dnmt 3L; DNA Cytosine 5' Methyltransferase 3 Like Protein); Anti -DNA Methyltransferase 3L (Dnmt 3L; anti-DNA Methyltransferase 3L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes Human DNMT3L MW
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Applicable Applications for anti-DNA Methyltransferase 3L antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Dilution: ELISA: 1:1000-1:3000
Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells
(10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot.
Dilution: ELISA: 1:1000-1:3000
Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells
(10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot.
Immunogen
A synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-DNA Methyltransferase 3L antibody
DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have
Product Categories/Family for anti-DNA Methyltransferase 3L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
DNA-methyltransferase
Similar Products
Product Notes
The DNA Methyltransferase 3L (Catalog #AAA616893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5' Methyltransferase 3 Like Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA Methyltransferase 3L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Immunoprecipitation and Western Blot. Dilution: ELISA: 1:1000-1:3000 Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells (10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot. Researchers should empirically determine the suitability of the DNA Methyltransferase 3L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Methyltransferase 3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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