Rabbit anti-Human DLL3 Polyclonal Antibody | anti-DLL3 antibody

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (Biotin)

Cat. Num. AAA6292368

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DLL3; Polyclonal Antibody; CT (DLL3; Delta-like protein 3; Drosophila Delta homolog 3) (Biotin); Drosophila Delta homolog 3; anti-DLL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DLL3 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526~556 amino acids from the C-terminal region of human DLL3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DLL3 Antibody (C-term) in A375 cell line lysates (35ug/lane). DLL3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DLL3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DLL3 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-DLL3 antibody

DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

Product Categories/Family for anti-DLL3 antibody

Antibodies; Notch Proteins

References

Yerges, L.M. J. Bone Miner. Res. 24 (12), 2039-2049 (2009). Heuss, S.F. Proc. Natl. Acad. Sci. U.S.A. 105 (32), 11212-11217 (2008). Maisenbacher, M.K. Hum. Genet. 116 (5), 416-419 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 45243561
• NCBI GeneID: 10683
• NCBI Accession #: NP_982353.1
• NCBI GenBank Nucleotide #: NM_203486.2
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 61,178 Da
• NCBI Official Full Name: delta-like protein 3 isoform 2
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 3
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm ().