Mouse Circadian Locomoter Output Cycles Protein Kaput ELISA Kit | CLOCK elisa kit

Mouse Circadian Locomoter Output Cycles Protein Kaput (CLOCK) ELISA Kit

Cat. Num. AAA2033691

• Gene Names: CLOCK; KAT13D; bHLHe8
• Reactivity: Mouse
• Synonyms: Circadian Locomoter Output Cycles Protein Kaput; Mouse Circadian Locomoter Output Cycles Protein Kaput (CLOCK) ELISA Kit; KAT13D; bHLHe8; Class E basic helix-loop-helix protein 8; CLOCK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Circadian Locomoter Output Cycles Protein Kaput (CLOCK).; No significant cross-reactivity or interference between Circadian Locomoter Output Cycles Protein Kaput (CLOCK) and analogues was observed.
• Sequence Length: 846

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for CLOCK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Circadian Locomoter Output Cycles Protein Kaput (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Circadian Locomoter Output Cycles Protein Kaput (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CLOCK elisa kit

Signal transduction; Circadian rhythm

NCBI and Uniprot Product Information

• NCBI GI #: 392841204
• NCBI GeneID: 9575
• NCBI Accession #: NP_001254772.1
• NCBI GenBank Nucleotide #: NM_001267843.1
• Molecular Weight: 95,304 Da
• NCBI Official Full Name: circadian locomoter output cycles protein kaput
• NCBI Official Synonym Full Names: clock circadian regulator
• NCBI Official Symbol: CLOCK
• NCBI Official Synonym Symbols: KAT13D; bHLHe8
• NCBI Protein Information: circadian locomoter output cycles protein kaput
• UniProt Protein Name: Circadian locomoter output cycles protein kaput
• Protein Family: Circadian locomoter output cycles protein
• UniProt Gene Name: CLOCK
• UniProt Synonym Gene Names: BHLHE8; KIAA0334; hCLOCK; bHLHe8
• UniProt Entry Name: CLOCK_HUMAN

NCBI Description

The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: Transcription factor; DNA-binding; Acetyltransferase; EC 2.3.1.48

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: chromosome; intracellular membrane-bound organelle; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; DNA binding; histone acetyltransferase activity; protein binding; protein dimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: activation of NF-kappaB transcription factor; circadian regulation of gene expression; circadian rhythm; DNA damage checkpoint; entrainment of circadian clock; establishment and/or maintenance of chromatin architecture; histone acetylation; negative regulation of transcription, DNA-dependent; photoperiodism; positive regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of hair cycle; regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to redox state; signal transduction; spermatogenesis; transcription from RNA polymerase II promoter