Rabbit anti-Human, Mouse DLGAP5 Polyclonal Antibody | anti-DLGAP5 antibody

DLGAP5 Polyclonal Antibody

Cat. Num. AAA3008300

• Gene Names: DLGAP5; DLG7; HURP
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DLGAP5; Polyclonal Antibody; DLGAP5 Polyclonal Antibody; DLG7; HURP; KIAA0008; anti-DLGAP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DLGAP5 polyclonal antibody detects endogenous levels of DLGAP5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 846

• Applicable Applications for anti-DLGAP5 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human DLGAP5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DLGAP5 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using DLGAP5 polyclonal antibody)

Related Product Information for anti-DLGAP5 antibody

DLG7 is a potential cell cycle regulator that may play a role in carcinogenesis of cancer cells and mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. The protein is also a key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 82592583
• NCBI GeneID: 9787
• UniProt Accession #: Q15398
• NCBI Official Full Name: Disks large-associated protein 5
• NCBI Official Synonym Full Names: DLG associated protein 5
• NCBI Official Symbol: DLGAP5
• NCBI Official Synonym Symbols: DLG7; HURP
• NCBI Protein Information: disks large-associated protein 5
• UniProt Protein Name: Disks large-associated protein 5
• Protein Family: Disks large-associated protein
• UniProt Gene Name: DLGAP5
• UniProt Synonym Gene Names: DLG7; KIAA0008; DAP-5; HURP
• UniProt Entry Name: DLGP5_HUMAN

Uniprot Description

DLG7: a cell cycle regulator that may play a role in carcinogenesis. Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. Required for chromosome congression and alignment. Stabilizes mitotic microtubules, promotes microtubule polymerization and controls spindle formation and dynamics Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Interacts with CDC2 and localizes to the spindle poles in mitotic cells. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Colocalizes with E-cadherin (CDH1) at sites of cell-cell contact in intestinal epithelial cells. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. Two alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Microtubule-binding

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein binding; phosphoprotein phosphatase activity

Biological Process: cell proliferation; dephosphorylation; cell-cell signaling; positive regulation of mitotic metaphase/anaphase transition; mitotic chromosome movement towards spindle pole