Rabbit anti-Human DLG7 Polyclonal Antibody | anti-DLG7 antibody

DLG7, ID (DLGAP5, DLG7, KIAA0008, Disks large-associated protein 5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein) (Biotin)

Cat. Num. AAA6292313

• Gene Names: DLGAP5; DLG7; HURP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: DLG7; Polyclonal Antibody; ID (DLGAP5; KIAA0008; Disks large-associated protein 5; Discs large homolog 7; Disks large-associated protein DLG7; Hepatoma up-regulated protein) (Biotin); DLGAP5; Hepatoma up-regulated protein; anti-DLG7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DLG7 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: DLG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-265 amino acids from the Central region of human DLG7.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DLG7 Antibody (Center) in 293 cell line lysates (35ug/lane).DLG7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DLG7 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DLG7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DLG7 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DLG7 antibody

DLG7 is potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. DLG7 is mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. DLG7 is key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

Product Categories/Family for anti-DLG7 antibody

Antibodies; KIAA; orf

References

de Reynies, A., et al. J. Clin. Oncol. 27(7):1108-1115(2009). Wong, J., et al. Mol. Biol. Cell 19(5):2083-2091(2008). Gudmundsson, K.O., et al. Stem Cells 25(6):1498-1506(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 82592583
• NCBI GeneID: 9787
• UniProt Accession #: Q15398; Q86T11; Q8NG58; A8MTM6; B4DRM8
• Molecular Weight: 846
• NCBI Official Full Name: Disks large-associated protein 5
• NCBI Official Synonym Full Names: discs, large (Drosophila) homolog-associated protein 5
• NCBI Official Symbol: DLGAP5
• NCBI Official Synonym Symbols: DLG7; HURP
• NCBI Protein Information: disks large-associated protein 5; DAP-5; discs large homolog 7; discs, large homolog 7; hepatoma up-regulated protein; disks large-associated protein DLG7
• UniProt Protein Name: Disks large-associated protein 5
• UniProt Gene Name: DLGAP5
• UniProt Synonym Gene Names: DLG7; KIAA0008; DAP-5; HURP
• UniProt Entry Name: DLGP5_HUMAN

Uniprot Description

DLG7: a cell cycle regulator that may play a role in carcinogenesis. Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. Required for chromosome congression and alignment. Stabilizes mitotic microtubules, promotes microtubule polymerization and controls spindle formation and dynamics Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Interacts with CDC2 and localizes to the spindle poles in mitotic cells. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Colocalizes with E-cadherin (CDH1) at sites of cell-cell contact in intestinal epithelial cells. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. Two alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Microtubule-binding

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm; microtubule organizing center; spindle pole centrosome; nucleus

Molecular Function: protein binding; phosphoprotein phosphatase activity

Biological Process: cell proliferation; dephosphorylation; cell-cell signaling; positive regulation of mitotic metaphase/anaphase transition; mitotic chromosome movement towards spindle pole