Immunoprecipitation (IP)
(DKK1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-DKK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DKK1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Rabbit anti-Human DKK1 Polyclonal Antibody | anti-DKK1 antibody
Anti-DKK1 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
Immunoprecipitation (IP)
(DKK1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-DKK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DKK1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Immunoprecipitation (IP)
(DKK1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-DKK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DKK1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Western Blot (WB)
(Anti-DKK1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:36 kDa)
Western Blot (WB)
(Anti-DKK1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:36 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]
Uniprot Description
DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.
Protein type: Secreted; Secreted, signal peptide; Inhibitor
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: extracellular space; early endosome membrane; extracellular region; plasma membrane
Molecular Function: protein binding; receptor antagonist activity; signal transducer activity; growth factor activity; low-density lipoprotein receptor binding
Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; mesoderm formation; hair follicle development; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis
Research Articles on DKK1
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Product Notes
The DKK1 dkk1 (Catalog #AAA8101472) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DKK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DKK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the DKK1 dkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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