Rabbit anti-Human, Mouse ABCF1 Polyclonal Antibody | anti-ABCF1 antibody

ABCF1, CT (ABCF1, ABC50, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, TNF-alpha-stimulated ABC protein) (PE)

Cat. Num. AAA6270517

• Gene Names: ABCF1; ABC27; ABC50
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ABCF1; Polyclonal Antibody; CT (ABCF1; ABC50; ATP-binding cassette sub-family F member 1; ATP-binding cassette 50; TNF-alpha-stimulated ABC protein) (PE); TNF-alpha-stimulated ABC protein; anti-ABCF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ABCF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ABCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 694~724 amino acids from the C-terminal region of human ABCF1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABCF1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). ABCF1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ABCF1 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). ABCF1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ABCF1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ABCF1 antibody

The protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process.

Product Categories/Family for anti-ABCF1 antibody

Antibodies; Multi Drug Resistance (MDR)

References

Barcellos, L.F., et.al., PLoS Genet. 5 (10), E1000696 (2009). Paytubi, S., et.al., J. Biol. Chem. 284 (36), 24061-24073 (2009). Saito, A., et.al., J. Hum. Genet. 54 (6), 317-323 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 69354671
• NCBI GeneID: 23
• NCBI Accession #: NP_001020262.1
• NCBI GenBank Nucleotide #: NM_001025091.1
• UniProt Accession #: Q8NE71; O14897; Q69YP6; A2BF75
• Molecular Weight: 95,926 Da
• NCBI Official Full Name: ATP-binding cassette sub-family F member 1 isoform a
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family F (GCN20), member 1
• NCBI Official Symbol: ABCF1
• NCBI Official Synonym Symbols: ABC27; ABC50
• NCBI Protein Information: ATP-binding cassette sub-family F member 1; TNF-alpha-stimulated ABC protein; TNFalpha-inducible ATP-binding protein; ATP-binding cassette 50 (TNF-alpha stimulated)
• UniProt Protein Name: ATP-binding cassette sub-family F member 1
• Protein Family: ABC transporter F family
• UniProt Gene Name: ABCF1
• UniProt Synonym Gene Names: ABC50
• UniProt Entry Name: ABCF1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCF1: Isoform 2 is required for efficient Cap- and IRES- mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; ribosome; nuclear envelope

Molecular Function: protein binding; translation activator activity; translation factor activity, nucleic acid binding; ATPase activity; ribosome binding; ATP binding

Biological Process: positive regulation of translation; translation; ribosome biogenesis and assembly; translational initiation; inflammatory response